摘要
采用极性不同的6种溶剂(石油醚、乙酸乙酯、丙酮、乙醇、甲醇和水)、按索氏提取法逐级萃取破壁灵芝孢子粉,并同时运用气相色谱-质谱联用(GC/MS)和超高效液相串联四极杆飞行时间质谱(UPLC-QTOF/MS)技术对各萃取物进行化学成分分析与鉴定。结果表明:GC/MS共鉴定出101种化合物,其中酸类10种、酯类40种、醇类7种、酮类6种、酚类2种、烃类18种、甾类9种和杂原子化合物9种;UPLC-Q-TOF/MS共推断出40种化合物,其中倍半萜类1种、二萜类1种、三萜类9种、生物碱类4种、酰胺类7种、有机酸类9种以及其他化合物9种。两种测定方法间共有化合物仅1种,仅存在于5种有机溶剂(石油醚、乙酸乙酯、丙酮、乙醇和甲醇)萃取物之一的化合物共105种,2种或2种以上萃取物共有的化合物共31种,实验方法较好地实现了样品中化合物组分的充分分离,扩大了可检测化合物的范围。研究结果为灵芝孢子粉中化学成分的系统分析与鉴定、及灵芝孢子粉的化合物谱图库的完善提供了基础资料,为相关药理、药效分析及灵芝的药用模式真菌研究提供参考。
Sporoderm-broken Ganoderma lingzhi spore powder was extracted fractionally with soxhlet extraction method using petroleum ether,ethyl acetate,acetone,ethanol,methanol and water as solvents with different proper polarity,and six extracts were obtained.The extracts were analyzed with gas chromatography-mass spectrometry(GC/MS)and ultra-performance liquid chromatography coupled with electrospray time-of-flight/mass spectrometry(UPLC-QTOF/MS).A total of 101 compounds is identified,including 10 acids,40 esters,7 alcohols,6 ketones,2 phenols,18 hydrocarbons,9 steroids and 9 heteroatomcontaining compounds.40 compounds are detected using UPLC-QTOF/MS,including 1 sesquiterpene,1 diterpenoid,9 triterpenes,4 alkaloids,7 amides,9 organic acids and 9 other compounds.Only 1 compound is in common among those obtained by GC/MS and UPLC-QTOF/MS analyses.105 compounds strickly present in their own selective extract and not reappear in other unselective one,but there are 31 compounds which can simultaneously present in two or more extracts.The method is proved to be better for separating the multi-component composition and widening the detection range of the compounds in Ganoderma lingzhi spore powder.
作者
佘新松
姚婷
韩燕峰
甘卓亭
周蔚
袁旺生
唐敏
SHE Xin-Song;YAO Ting;HAN Yan-Feng;GAN Zhuo-Ting;ZHOU Wei;YUAN Wang-Sheng;TANG Min(College of Life and Environment Science,Huangshan University,Huangshan,Anhui 245041,China;Analysis and Test Center,Huangshan University,Huangshan,Anhui 245041,China;College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China;School of Tourism,Huangshan University,Huangshan,Anhui 245041,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第5期881-906,共26页
Mycosystema
基金
国家自然科学基金(31460010)
安徽省教育厅自然科学研究重点项目(KJ2019A0614)
校级专项资助项目(2018XZX001)
国家级大学生创新创业训练计划项目(2019)。
关键词
灵芝孢子粉
气相色谱-质谱联用
超高效液相串联四极杆飞行时间质谱
分级萃取
Ganoderma lingzhi spore powder
gas chromatography-mass spectrometry
ultra-performance liquid chromatography coupled with electrospray time-of-flight/mass spectrometry
fractional extraction
