摘要
[目的]探究具有防治土传病害和促进植物生长功能的3株土壤链霉菌对谷子白发病的防治潜能及对谷子植株生长的影响。[方法]通过室内盆栽试验,谷子种子分别包衣3株土壤链霉菌黄白链霉菌(Streptomyces albidoflavus)T4、密旋链霉菌(Streptomyces pactum)Act12和娄彻氏链霉菌(Streptomyces rochei)D74的活菌制剂,同时以无菌剂包衣的处理作为对照,于播种后15 d、45 d和75 d统计谷子白发病病叶数量,并对播种后75 d发病叶片进行分级,计算病情指数;同时测定各时期谷子生长相关指标。[结果]播种后75 d,T4、Act12和D74菌剂处理的谷子病叶率与不施加菌剂的对照相比分别降低了41.4%、20.6%和18.7%(P<0.05);T4和D74菌剂处理的谷子白发病的病情指数与对照相比分别降低了36.0%和21.3%(P<0.05);T4、Act12和D74菌剂处理的谷子其播种后75 d地上部干重与对照相比分别增加了32.9%、23.7%和22.7%(P<0.05)。此外,T4还增加了苗期谷子株高及播种后75 d叶片数。[结论]包衣施加T4、Act12和D743株链霉菌对接菌早期谷子白发病的防治作用不大,但抑制了后期(播种后75 d)白发病的发病程度并促进了谷子植株生长,其中以T4的防病促生效果最强。本研究表明,土壤中兼具抗植物土传病害和促进植物生长功能的链霉菌是谷子白发病的潜在生防菌库,可从中筛选谷子白发病的有效防治菌株。
[Objective]The objective of this research was to explore the potential control effects of three soil actinomycetes with the function of preventing soil-borne plant pathogens and promoting plant growth on the occurrence of millet downy mildew and the influence on the growth of millet plants.[Methods]Three soil actinomycetes strains Streptomyces albidoflavus T4,Strep⁃tomyces pactum Act12,and Streptomyces rochei D74 were used in this research in the form of dry powder.The millet seeds were coated with the living actinomycetes agents,and sown in pots under laboratory condition.The untreated seeds were considered as the control.The number of diseased leaves was counted at 15,45 and 75 days after sowing,and the diseased level was graded at 75 days after sowing to calculate disease index.The plant growth indexes were also measured at each sampling time to investigate the control effects on millet downy mildew and growth promotion effects on millet plants of the tested three actinomycetes strains.[Results]At 75 days after sowing,T4,Act12,and D74 reduced the diseased leaf proportion,by 41.4%,20.6%,and 18.7%,respectively(P<0.05)compared with that of the control;T4 and D74 also decreased the disease index by 36.0%and 21.3%in that order(P<0.05).also increased the dry biomass of aboveground part of millet plants were increased by 32.9%,23.7%,and 22.7%in T4,Act12,and D74 treatments,respectively(P<0.05)at 75 days after sowing.In addition,T4 treatment resulted in the increase of plant height and total number of millet leaves.[Conclusion]T4,Act12,and D74 had limited effect on the control of millet downy mildew at the early stage,but inhibited the disease degree later on(75 days after sowing)and promoted millet plant growth.Among the three tested strains,T4 showed the strongest effects.Soil Streptomyces that can inhibited soil borne disease and promote plant growth may also be effective as biocontrol agents for millet downy mildew control,and active biocontrol strains can be screened out from these Streptomyces.
作者
陈杰
杨珍平
夏清
陈昆仑
高志强
韩彦卿
韩渊怀
林文
Chen Jie;Yang Zhenping;Xia Qing;Chen Kunlun;Gao Zhiqiang;Han Yanqing;Han Yuanhuai;Lin Wen(College of Agriculture,Shanxi Agricultural University,Taigu 030801,China)
出处
《山西农业大学学报（自然科学版）》
CAS
北大核心
2020年第4期82-88,共7页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
山西省优秀博士来晋奖励项目(SXYBKY201701)
山西农业大学科技创新项目(2019002)
山西农业大学博士科研启动项目(2017YJ24)。
关键词
谷子
白发病
放线菌
链霉菌
生物防治
Millet
Downy mildew
Actinomycetes
Streptomyces
Biocontrol
