摘要
从土壤中分离获得1株具有较强拮抗作用的细菌菌株X-42,经形态特征、16S rDNA序列分析,将其鉴定为解淀粉芽孢杆菌。该菌可产生一系列具有抑菌功能的代谢物,抑制禾谷镰刀菌生长。从X-42菌株发酵液中分离纯化代谢物,经硫酸铵沉淀的脂肽类物质用于抑菌分析,结果表明,来自X-42的脂肽物,既能直接破坏禾谷镰刀菌菌丝结构,也能影响分生孢子萌发,导致菌丝及孢子局部膨大。小麦开花期的田间赤霉病防治研究表明,在接种禾谷镰刀菌前和接种后施用拮抗菌X-42代谢物,均可有效防治赤霉病,其防治效果高达79%～88%,与化学杀菌剂多菌灵相当。
One antagonistic bacterial strain was isolated form soli and identified as Bacillus amyloliquefaciens X-42 based on its morphology and 16S rDNA sequences. This strain produced a series of metabolites that exhibited a strong inhibitory activity to Fusarium grarninearum. From its secreted metabolites during fermentation, some lipopeptides were precipitated with ammonia sulfate and used for antifungal assays. These lipopeptides were able to directly damage mycelia and conidiospores of F. gra- minearurn,resulting in severely deformed, partly enlarged structures. Field assays showed a significant reduction of Fusarium head blight （FHB） diseases on wheat when X-42 suspension was sprayed on wheat spikes either before or after inoculation with F. graminearum. Relative protective efficacies ranged from 79% to 88~, comparable to that treated with chemical fungicide carbendazim. This study may serve as foundation for the development of new and environmental friendly biological agents to control FHB.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2013年第3期28-32,共5页
Journal of Huazhong Agricultural University
基金
国家重点基础研究发展计划"973"项目(2009CB118806)
国家转基因专项(2011ZX08002-001)资助
关键词
拮抗
解淀粉芽孢杆菌
生物防治
禾谷镰刀菌
小麦赤霉病
antagonism
Bacillus amyloliquefaciens
biological control
Fusarium graminearum Fusarium head blight
