摘要
目的探讨菊粉(INU)对非酒精性脂肪肝病(NAFLD)小鼠单核细胞型髓源性抑制细胞(M-MDSC)和血浆炎症因子的影响。方法C57BL/6小鼠随机分为正常饮食组、INU对照组、NAFLD模型组和INU处理的NAFLD模型组。正常饮食组和INU对照组给予普通饲料,NAFLD模型组和INU处理的NAFLD模型组给予高脂饲料(含60%脂肪)。同时INU对照组和INU处理的NAFLD模型组给予INU(5 g/kg),喂养14周后,处死小鼠并收集小鼠外周血、肝脏和脾脏细胞。采用流式细胞术检测外周血、肝脏和脾脏中M-MDSC比例。细胞因子流式微球阵列检测技术(CBA)检测血浆中肿瘤坏死因子α(TNF-α)和白细胞介素10(IL-10)表达水平。Pearson统计分析各组织M-MDSC比例和血浆炎症因子水平的相关性。结果与正常饮食组相比,NAFLD模型组外周血、肝脏和脾脏中M-MDSC的比例明显增加,血浆中TNF-α水平显著升高,IL-10水平变化不明显。与NAFLD模型组相比,INU处理的NAFLD模型组外周血、肝脏和脾脏中M-MDSC的比例明显增加,TNF-α水平显著降低、IL-10水平显著升高。NAFLD小鼠外周血、肝脏及脾脏中M-MDSC分别与TNF-α呈负相关,与IL-10呈正相关。结论INU可募集NAFLD小鼠外周血、肝脏及脾脏中M-MDSC,发挥抗炎作用,延缓NAFLD的发生发展。
Objective To investigate the effect of inulin on monocytic myeloid-derived suppressor cells(M-MDSCs)and plasma inflammatory cytokines in mouse models with non-alcoholic fatty liver disease(NAFLD).Methods C57BL/6 mice were randomly divided into 4 groups:normal diet group,inulin-treated control group,NAFLD model group and inulin-treated NAFLD model group.The normal diet group and inulin-treated control group were given normal diet daily,while the other two groups were fed high-fat diet(60%fat)instead.Meanwhile,the mice in the two inulin-treated groups were administrated dietary inulin(5 g/kg).After 14 weeks of feeding,the mice were sacrificed and their peripheral blood,liver and spleen cells were collected.The proportion of M-MDSCs in the peripheral blood,spleen and liver cells were separately detected by flow cytometry.Concentrations of plasma inflammatory cytokines including tumor necrosis factorα(TNF-α)and interleukin 10(IL-10)were determined by flow cytometry CBA.Statistical Pearson was used to analyze the correlation between the proportion of M-MDSCs and plasma inflammatory cytokines.Results Compared with the normal diet group,the proportion of M-MDSCs in the peripheral blood,liver and spleen of the NAFLD model group significantly increased,plasma TNF-αlevel also dramatically increased,but IL-10 concentration showed no significant difference from that of the normal diet group.Conversely,INU treatment,compared with the NAFLD model group without INU treatment,increased the proportion of M-MDSCs in the peripheral blood,liver and spleen notably,decreased plasma TNF-αlevel,and increased plasma IL-10 concentration.The proportion of M-MDSCs in the peripheral blood,liver and spleen cells of NAFLD mice were negatively correlated with plasma TNF-αlevel,but positively correlated with plasma IL-10 concentration.Conclusion INU may alleviate the progression of NAFLD via recruiting M-MDSCs in the peripheral blood,liver and spleen and reducing inflammation in mice.
作者
鲍婷
王振
朱丽丽
卢海霞
汪婷
张艳婷
张晓霞
王浩
杨少奇
BAO Ting;WANG Zhen;ZHU Lili;LU Haixia;WANG Ting;ZHANG Yanting;ZHANG Xiaoxia;WANG Hao;YANG Shaoqi(Clinical Medical College,Ningxia Medical University,Yinchuan 750004,China;Department of Gastroenterology,General Hospital,Ningxia Medical University,Yinchuan 750004,China;Department of Pathogenic Biology and Medical Immunology,School of Basic Medical Sciences,Ningxia Medical University,Yinchuan 750004,China;College of Chinese Traditional Medicine,Ningxia Medical University,Yinchuan 750004,China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2020年第3期228-235,共8页
Chinese Journal of Cellular and Molecular Immunology
基金
宁夏“十三五”重大科技项目(2016BZ02)
宁夏医科大学优势学科群建设科研项目(XY201510)。
