摘要
目的研究EZH2对鼻咽癌血管生成拟态的影响及可能机制.方法慢病毒载体pLVTHM建立空载体对照(Lv-vector)、稳定过表达EZH2(Lv-EZH2)和沉默EZH2(Lv-shEZH2)的鼻咽癌细胞株;检测EZH2对鼻咽癌细胞增殖、迁移和血管生成拟态的影响;检测EZH2调控的下游相关的靶基因的表达.结果相比空载体组(Lv-vector),Lv-EZH2组细胞生长速度、相对移动距离、穿过聚碳酸酯膜的细胞数、单位面积内成管数、靶基因(ET-1、NOTCH4、CXCL5、HPSE)表达显著增加,而靶基因(PLAU、KDR、THBS1)表达显著降低(P<0.05);Lv-shEZH2组细胞生长速度、相对移动距离、穿膜的细胞数、单位[面积内成管数、靶基因(ET-1、NOTCH4、CXCL5、HPSE)表达显著降低,而靶基因(PLAU、KDR、THBS1)表达显著增加(P<0.05).结论EZH2可促进鼻咽癌细胞体外的增殖、迁移和血管生成拟态,机制与多个下游基因调控有关.
Objective To explore the effect of EZH2 on vasculogenic mimicry of nasopharyngeal carcinoma(NPC)and its possible mechanism.Methods Lentiviral vector(pLVTHM)was used to establish NPC cell lines with empty vector control(LV-vector),stably overexpressed EZH2(LV-EZH2)and silencing EZH2(LV-shEZH2).The effects of EZH2 on the proliferation,migration and vasculogenic mimicry of NPC cells were detected and the expression of downstream target genes regulated by EZH2 was detected.Results Compared with LV-vector group,LV-EZH2 group showed significantly increased cell growth rate,relative moving distance,number of cells passing through polycarbonate membrane,number of tube formation per unit area and the expression of target genes ET-1,NOTCH4,CXCL5 and HPSE,and significantly decreased expression of target genes PLAU,KDR and THBS1(P<0.05);LV-shEZH2 group showed significantly decreased cell growth rate,relative moving distance,number of cells passing through polycarbonate membrane,number of tube formation per unit area and the expression of target genes ET-1,NOTCH4,CXCL5 and HPSE,and significantly increased expression of target genes PLAU,KDR and THBS1(P<0.05).Conclusion EZH2 can promote the proliferation,migration and vasculogenic mimicry of NPC cells in vitro,and its mechanism is correlated with the regulation of several downstream genes.
作者
苗洪宾
黄宇
夏晨曦
冯华君
许胜恩
覃纲
赵飞鹏
Miao Hongbin;Huang Yu;Xia Chenxi;Feng Huajun;Xu Sheng'en;Qin Gang;Zhao Feipeng(Department of Otorhinolaryngology,Head and Neck Surgery,The Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan,646000,China)
出处
《西南国防医药》
CAS
2020年第6期513-518,共6页
Medical Journal of National Defending Forces in Southwest China
基金
国家自然科学基金项目(81602570)。
关键词
EZH2
鼻咽癌
血管生成拟态
分子机制
EZH2
nasopharyngeal carcinoma
vasculogenic mimicry
molecular mechanism
