摘要
目的观察柴胡三参胶囊对缺血性心律失常大鼠心肌细胞Bcl-2及Fas蛋白的表达影响,进一步明确柴胡三参胶囊治疗缺血性心律失常的作用及机制。方法将实验大鼠随机分为假手术组、模型组、柴胡三参胶囊组、参松养心胶囊组、维拉帕米组、空白组,每组各10只。给予相应药物干预2周后观察各组的心律失常积分和Bcl-2、Fas表达的变化。结果柴胡三参胶囊组、参松养心胶囊组、维拉帕米组与模型组进行比较,心律失常评分下降(P <0.05),Bcl-2蛋白表达升高(P <0.01),Fas蛋白表达有一定程度降低(P <0.05)。结论柴胡三参胶囊能上调抑制凋亡因子Bcl-2表达,下调促进凋亡因子Fas表达,说明其可减轻细胞凋亡,缓解心肌损伤,为柴胡三参胶囊改善缺血性心律失常提供了实验依据。
Objective:To observe the effect of Chaihu Sanshen Capsule on the expression of Bcl-2 and Fas protein in myocardial cells of rats with ischemic arrhythmia and to clarify the effect and mechanism of Bupleurum Sanshen capsule in the treatment of ischemic arrhythmia.Methods:The experimental rats were randomly divided into sham operation group,model group,Chaihu Sanshen Capsule group,Shensong Yangxin Capsule group,verapamil group,blank group,10 in each group.The changes of arrhythmia scores and Bcl-2,fas expression in the six groups were observed.Results:Compared with the model group,the arrhythmia scores of Chaihu Sanshen Capsule group,Shensong Yangxin Capsule group and verapamil group decreased significantly(P<0.05).The expression of Bcl-2 protein was increased,the drug intervention was effective,and the difference was statistically significant(P<0.01).Compared with the model group,the expression of Fas protein was reduced to a certain extent,and the difference was statistically significant(P<0.05).Conclusion:Chaihu Sanshen Capsule up-regulates and inhibits the expression of apoptosis factor Bcl-2,down-regulates and promotes the expression of apoptosis factor Fas,indicating that it can alleviate apoptosis and relieve myocardial injury,which provides experimental basis for Chaihu Sanshen Capsule to improve ischemic arrhythmia.
作者
刘建和
毛宗裕
肖冰凌
袁恒佑
杨成龙
赵吉锐
Liu Jianhe;Mao Zongyu;Xiao Bingling;Yuan Hengyou;Yang Chenglong;Zhao Jirui(The First Affiliated Hospital of Hunan University of Chinese Medicine,Hunan,Changshu 410007,China)
出处
《中国中医急症》
2020年第6期962-965,977,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
湖南省中医药科研计划项目(201703)
湖南省自然科学基金科卫联合项目(2018JJ6038)
湖南省学位与研究生教育改革研究项目(2019JGYB163)
湖南中医药大学中医学国内一流建设学科。
