摘要
目的探讨YAP蛋白和上皮间质转化(EMT)相关蛋白E-cadherin、Vimentin在宫颈鳞状细胞癌的表达及临床意义。方法采用免疫组化法检测20例正常宫颈、50例鳞状上皮内病变及125例宫颈鳞状细胞癌组织中YAP和EMT相关蛋白E-cadherin、Vimentin的表达,并分析其与临床病理特征的关系。结果在宫颈鳞状细胞癌组织中YAP、E-cadherin和Vimentin阳性表达率分别为71.20%、55.20%和48.80%,与鳞状上皮内病变和正常宫颈组织比较,差异有统计学意义(P<0.001);YAP蛋白的表达与组织学分级及淋巴结转移有关(P<0.05);E-cadherin及Vimentin蛋白的表达与组织学分级、FIGO分期及淋巴结转移有关(P<0.05),与其它临床病理参数无关(P>0.05);YAP的表达与E-cadherin的表达呈负相关关系(r=-0.395,P<0.05),而与Vimentin的表达呈正相关关系(r=0.338,P<0.001)。结论YAP蛋白的表达可能与宫颈鳞状细胞癌的发生发展有关,可能通过调控EMT促进宫颈鳞状细胞癌的浸润转移。
Objective To evaluate the expression of YAP protein and epithelial mesenchymal transition(EMT)associated proteins E-cadherin and Vimentin in cervical squamous cell carcinoma and its clinical significance.Methods Expression level of YAP protein and EMT associated proteins E-cadherin and Vimentin proteins in cervical carcinoma were detected by immunohistochemical method in 20 normal cervix,50 cases SIL and 125 cases cervical squamous cell carcinoma.The relation with clinical pathologic parameters was analyzed.Results The positive expression rate of YAP,E-cadherin and Vimentin were 71.20%,55.20%and 48.80%,Compared with SIL and normal cervical tissue(P<0.05).YAP expression was significant correlation with the degree of histological grade and lymph node metastasis(P<0.05),E-cadherin and Vimentin expression were significant correlation with the degree of histological grade,FIGO stage and lymph node metastasis(P<0.05),but there was not obviously correlated with other clinical pathological parameters(P>0.05).YAP expression was negative correlated with E-cadherin(r=0.395,P<0.05),YAP expression was positive correlated with Vimentin(r=0.338,P<0.05).Conclusion The expression of YAP protein may be related to the occurrence and development of cervical squamous cell carcinoma,and may promote the invasion and metastasis of cervical squamous cell carcinoma by regulating EMT.
作者
唐波
梁静
甘中华
王洪飞
高杰
王茂霞
孙玲
TANG Bo;LIANG Jing;GAN Zhonghua;WANG Hongfei;GAO Jie;WANG Maoxia;SUN Lingi(Department of Pathology, Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University, Luzhou 646000, Sichuan, China;Department of Ultrasound, Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University, Luzhou 646000, Sichuan, China)
出处
《西部医学》
2020年第6期823-826,共4页
Medical Journal of West China
