摘要
目的:明确低氧对恶性黑素瘤A375细胞中Nodal及细胞迁移相关蛋白表达的影响。方法:体外培养A375细胞,CoCl 2构建细胞内低氧模型。细胞分为空白对照组,CoCl 2组,SB-431542(Nodal抑制剂)组和CoCl 2+SB-431542组。通过Western Blot技术检测Nodal、类激活素激酶4/7(ALK4/7)、与恶性肿瘤进展相关的上皮钙黏附蛋白(E-cadherin)、基质金属蛋白酶-2(MMP 2)和波形蛋白(Vimentin)的表达水平。结果:CoCl 2组细胞内Nodal及ALK7蛋白浓度,高于其他组(P<0.05)。各处理组均没有检测到ALK4蛋白;CoCl 2组细胞中Vimentin和MMP2蛋白的浓度高于其他组(P<0.05);各处理组E-cadherin蛋白水平差异不具统计学意义。结论:低氧环境可诱导A375细胞中Nodal和细胞迁移相关蛋白MMP2和Vimentin的高表达。
Objective:To determine the effect of hypoxia on the expression of Nodal and the impact of relative migration protein in malignant melanoma A375 cell.Methods:Malignant melanoma A375 cells were cultured in vitro and treated with chloride(CoCl2)to construct an intracellular hypoxia model.The cells were divided into blank control group CoCl2 group,SB-431542 group and CoCl2+SB-431542 group.The level of Nodal,Activin receptor-like kinase 4/7(ALK4/7),E-cadherin,matrix metalloproteinase-2(MMP-2)and vimentin was detected by Western blot.Results:The level of Nodal and ALK7 in A375 cells was higher in CoCl2 group than in other groups,with significant differences(Ps<0.05).ALK4 was not detected in all groups.The level of Vimentin and MMP2 in A375 cells was higher in CoCl2 group than in other groups,with significant differences(Ps<0.05).There was no significant difference of E-cadherin protein in different groups(P>0.05).Conclusion:Hypoxia can increase the expression of Nodal and relative migration protein of MMP2 and vimentin in the malignant melanoma A375 cells.
作者
谭自敏
杨成莲
钟桂书
曾凡才
TAN Zimin;YANG Chenglian;ZHONG Guishu;ZENG Fancai(Department of Dermatology,The Affiliated Hospital of Southwest Medical University,Luzhou 646000,China;Department of Dermatology,Mianyang Third People's Hospital,Mianyang 621000,China;Department of Biochemistr,Southwest Medical University,Luzhou 646000,China)
出处
《中国麻风皮肤病杂志》
2020年第6期339-342,共4页
China Journal of Leprosy and Skin Diseases
