摘要
目的探讨健脾化痰方治疗高脂血症脾虚痰浊证的可能作用机制。方法普通级广西巴马小型猪15只随机分为正常组、模型组和给药组,每组5只。正常组予基础饲料喂饲,模型组和给药组均予高脂饲料喂饲24周建立高脂血症脾虚痰浊证小型猪模型。给药组在高脂饲料喂饲同时给予健脾化痰方颗粒剂,将颗粒剂溶于100ml生理盐水中,均匀掺入饲料内,每日1剂,喂饲24周。在0周和24周时采集小型猪空腹耳静脉血检测血清内皮功能指标包括一氧化氮(NO)、内皮型一氧化氮合酶(eNOS)、诱导型一氧化氮合酶(iNOS)、总一氧化氮合酶(tNOS),及血清内皮素1(ET-1)、细胞间黏附分子1(ICAM-1)水平;24周时检测冠状动脉内皮组织匀浆中内皮功能指标及ET-1、ICAM-1水平。结果24周时,与对照组比较,模型组小型猪血清和冠状动脉内皮组织匀浆中tNOS、iNOS、ET-1、ICAM-1水平均升高,eNOS、NO水平降低(P<0.05);与模型组比较,给药组小型猪血清和冠状动脉内皮组织匀浆中tNOS、iNOS、ET-1、ICAM-1水平均降低,eNOS、NO水平升高(P<0.05)。结论健脾化痰方能有效改善血管内皮功能,可能是其治疗高脂血症脾虚痰浊证的作用机制之一。
Objective To explore the possible mechanism of Jianpi Huatan Formula(健脾化痰方)in the treatment of hyperlipidemia with spleen deficiency and phlegm turbid syndrome.Methods A total of 15 ordinary Guangxi Bama minipigs were randomly divided into a normal group,a model group and a treatment group,with 5 in each group.The normal group was fed with basic feed,the model group and the treatment group were fed with high-fat feed for 24 weeks to establish a small pig model of hyperlipidemia with spleen deficiency and phlegm turbidity syndrome.The treatment group was given Jianpi Huatan Formula Granules(健脾化痰方颗粒剂)at the same time given high-fat feed.The granules were dissolved in 100 ml of normal saline and evenly mixed into the feed.One dose per day for 24 weeks.At 0 week and 24 weeks,fasting auricular venous blood of the minipigs was collected to detect the levels of nitric oxide(NO),endothelial nitric oxide synthase(eNOS),inducible nitric oxide synthase(iNOS),total nitric oxide synthase(TNOs),serum endothelin 1(ET-1)and intercellular adhesion molecule 1(ICAM-1).After 24 weeks,the endothelial function index and ET-1,ICAM-1 level in endothelium homogenate of coronary artery was measured.Results At 24 weeks,compared with the control group,the levels of tNOS,iNOS,ET-1,ICAM-1 were increased,and the levels of eNOS,NO were decreased in the model group(P<0.05).Compared with the model group,the levels of tNOS,iNOS,ET-1,ICAM-1 in minipig serum and coronary artery endothelial tissue homogenate were decreased,and the levels of eNOS and NO were increased(P<0.05).Conclusion Jianpi Huatan Formula(健脾化痰方)can effectively improve the function of endothelium,which may be one of the mechanisms of its treatment of hyperlipidemia with spleen deficiency and phlegm turbid syndrome.
作者
李芹
张会永
周鹤
庞琳琳
李佳
肖程予
杨关林
LI Qin;ZHANG Huiyong;ZHOU He;PANG Linlin;LI Jia;XIAO Chengyu;YANG Guanlin(Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang,110032;Liaoning University of Traditional Chinese Medicine)
出处
《中医杂志》
CSCD
北大核心
2020年第10期886-889,895,共5页
Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81603513)
辽宁中医药大学附属医院院内项目(2017Y2)。
关键词
高脂血症
脾虚痰浊证
健脾化痰方
血管内皮功能
一氧化氮合酶
hyperlipidemia
spleen deficiency and phlegm turbid syndrome
Jianpi Huatan Formula健脾化痰方
endothelial function
nitric oxide synthetase
