摘要
目的:观察比较血府逐瘀汤和加味二陈汤对高脂饮食诱导的载脂蛋白E敲除(ApoE-/-)小鼠非酒精性脂肪肝(NAFLD)的改善作用及机制。方法:10只C57/BL6J小鼠为正常组,以一般饲料喂养,30只ApoE-/-小鼠为造模组,以高脂饲料喂养4周,后分为模型组、血府逐瘀汤组(以下简称为活血组)和加味二陈汤组(以下简称为化痰组),每组10只。正常组及模型组灌胃生理盐水,给药组以成人剂量的10倍予以灌胃,每天1次,共8周,于12周实验结束后取小鼠血清和肝脏。蛋白免疫印迹法(Western blot)与实时荧光定量聚合酶链式反应(Real-time PCR)测定各组血清血脂和肝功水平,观察肝脏病理形态,检测肝脏炎症介质白细胞介素-1β(IL-1β),肿瘤坏死因子-α(TNF-α),基质金属蛋白酶-9(MMP-9),单核细胞趋化因子-1(MCP-1)蛋白和mRNA表达水平。结果:血清血脂与肝功结果显示,与正常组比较,模型组小鼠血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白(LDL),天门冬氨酸氨基转移酶(AST),丙氨酸氨基转移酶(ALT)显著升高,高密度脂蛋白(HDL)显著降低(P<0.01);与模型组比较,活血组小鼠血清TG,LDL,ALT明显降低,HDL明显升高(P<0.05);化痰组小鼠血清TC,TG,LDL,AST,ALT明显降低,HDL明显升高(P<0.05,P<0.01),其中化痰组小鼠血清HDL升高幅度较活血组高,活血组小鼠血清ALT降低幅度较化痰组大。病理学观察显示,与正常组比较,模型组肝细胞脂肪变性严重,存在许多脂滴空泡,提示小鼠NAFLD模型成功;与模型组比较,各给药组均减轻肝细胞脂肪性变,两给药组间并无显著差异。Western blot与Real-time PCR结果显示,与正常组比较,模型组TNF-α,IL-1β,MCP-1,MMP-9的蛋白与mRNA表达水平明显升高(P<0.05,P<0.01);与模型组比较,活血组可明显下调IL-1β,MCP-1蛋白和MCP-1 mRNA的表达(P<0.05,P<0.01),化痰组可明显降低IL-1β,TNF-α,MMP-9,MCP-1蛋白和TNF-α,MMP-9 mRNA的表达(P<0.05,P<0.01)。结论:加味二陈汤和血�
Objective:To compare the effect and mechanisms of modified Erchentang and Xuefu Zhuyutang on high-fat diet-induced apolipoprotein-E knockout(ApoE-/-)mice nonalcoholic fatty liver disease(NAFLD).Method:Ten C57/BL6 J mice were taken as normal control group and fed with normal feed.Totally30 ApoE-/-mice were fed with high-fat diet to establish a disease model for 4 weeks.After 4 weeks,the 30 ApoE-/-mice were divided into model group,Xuefu Zhuyutang group(hereinafter referred to as Huoxue group)and modified Erchentang group(hereinafter referred to as Huatan group)by random number table method,with10 in each group.The normal group and the model group were intragastrically administered with normal saline.The drug-administered group was intragastrically administered at a dosage that was ten times of the adult dose,once a day,for 8 weeks.Serum and liver were collected after the end of the 12-week experiment.The serum lipid and liver function levels of each group were measured,and the liver pathological morphology was observed.Protein and mRNA expressions of liver inflammatory mediators interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),matrix metalloproteinase-9(MMP-9),monocyte chemotactic factor-1(MCP-1)were detected by Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR)and Western blot.Result:The results of serum lipids and liver function showed that compared with the normal group,serum total cholesterol(TC),triglyceride(TG),low-density lipoprotein(LDL),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)in the model group were significantly increased,while serum high-density lipoprotein(HDL)was significantly reduced(P<0.01).Compared with the model group,serum TG,LDL and ALT were significantly decreased,HDL was significantly increased in the Huoxue group(P<0.05).The serum levels of TC,TG,LDL,AST and ALT in the Huatan group were significantly decreased,HDL was significantly increased(P<0.05,P<0.01),and TG was decreased.The mice serum HDL in the Huatan group was higher than tha
作者
郭宏雅
付远飞
刘惠婷
王剑
GUO Hong-ya;FU Yuan-fei;LIU Hui-ting;WANG Jian(Basic Medical School,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Basic Research Center of integrated Chinese and Western Medicines,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第12期71-77,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(8187151662)。
