摘要
目的建立高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)测定鸡蛋中38种药物残留的分析方法。方法样品采用0.1 mol/L乙二胺四乙酸二钠(ethylene-diamine-tetra-acetate,EDTA)和乙腈提取,经QuEChERS净化后,通过LC-MS/MS测定,外标法定量。结果鸡蛋中38种药物在0.3~100μg/kg浓度范围内相关系数(r 2)在0.99138~0.99989之间,线性关系良好。方法检出限0.05~1.0μg/kg,定量限0.1~3.0μg/kg,在3.0、10.0、50.0μg/kg 3个添加水平的平均回收率72.49%~114.06%,相对标准偏差(n=6)2.37%~13.85%。结论本方法操作简便快速、灵敏稳定,适用于鸡蛋中多种药物残留的高通量快速检测。
Objective To establish a method for determination of 38 kinds of veterinary drugs in eggs by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Methods The sample was extracted with 0.1 mol/L EDTA and acetonitrile,and cleaned up by QuEChERS(quick,easy,cheap,effective,rugged,and safe)extraction method.Then determination of the analytes was performed by LC-MS/MS in negative and positive ionization mode using an electrospray ionization source adopting the multi-reaction monitoring mode.Quantitative was achieved by an external standard method.Results The correlation coefficient(r 2)of 38 kinds of drugs in eggs was 0.99138-0.99989 in the concentration range of 0.3-100μg/kg,and the linear relationship was good.Methods the detection limit was 0.05-1.0μg/kg,the quantitative limit was 0.1-3.0μg/kg.The average recoveries of the 38 veterinary drugs were ranged from 72.49%to 114.06%at 3 spiked levels of 3.0,10.0 and 50.0μg/kg,with the relative standard deviations(RSDs)in the range of 2.37%-13.85%.Conclusion The proposed method is simple,fast,sensitive and accurate,which is suitable for fast high-throughput detection of multiple residues in eggs.
作者
王娇
李庆霞
韵小娟
谢玉荣
杨信
WANG Jiao;LI Qing-Xia;YUN Xiao-Juan;XIE Yu-Rong;YANG Xin(Animal Disease Prevention and Control Center of Hainan Province,Haikou 570203,China)
出处
《食品安全质量检测学报》
CAS
2020年第8期2503-2510,共8页
Journal of Food Safety and Quality
基金
海南省自然科学基金青年基金项目(819QN393)。
