摘要
目的探讨低强度经皮迷走神经耳支刺激(AB-VNS)治疗对心衰犬心功能改善情况的影响。方法24只Beagle犬均植入起搏器,分成假手术组(n=8),心衰组(n=8),AB-VNS组(n=8)。假手术组仅植入起搏器但不起博;心衰组和AB-VNS组行起搏器起搏制作心衰模型,AB-VNS组给予低强度经皮耳迷走神经刺激治疗8周。心脏超声测量射血分数(EF)和左室舒张末期直径(LVEDD);酶联免疫吸附法测定血清NT-proBNP、CRP和TNF-α浓度;电生理方法检测心率、ERP;取心肌组织做Masson染色观察心肌组织形态学改变。结果经皮耳迷走神经刺激治疗8周后AB-VNS组的EF[(53.50±2.59)%]高于心衰组[(45.17±3.06)%],LVEDD[(37.17±1.60)mm]小于心衰组[(53.83±6.15)mm],差异有统计学意义(P<0.05);AB-VNS组Nt-proBNP低于心衰组[(388.56±40.94)pg/ml vs(674.91±56.78)pg/ml]、CRP[(9.57±1.18)mg/L vs(9.57±1.18)mg/L]、TNF-α[(6.40±0.63)ng/L vs(10.82±0.88)ng/L]、静息心率[(124.17±7.30)bpm vs(146.67±8.14)bpm],ERP较心衰组延长[(158.50±5.24)ms vs(125.17±4.83)ms],差异有统计学意义(P<0.05)。AB-VNS组Masson染色示心肌细胞形态较规则,伴有少量胶原纤维。结论低强度经皮迷走神经耳支刺激有助于提高心衰犬心射血分数、降低NT-proBNP和炎症因子,并减慢心率、延长有效不应期。
Objective To investigate the effect of low-intensity percutaneous vagal nerve branch stimulation(AB-VNS)on the improvement of heart function in dogs with heart failure.Methods All 24 Beagle dogs were implanted with pacemakers and divided into sham operation group(n=8),heart failure group(n=8),AB-VNS group(n=8).In the sham-operated group,only a pacemaker was implanted,but it was not worthwhile;the heart failure group and the AB-VNS group were paced to make a heart failure model,and the AB-VNS group was given low-intensity percutaneous ear vagus nerve stimulation treatment for 8 weeks.Cardiac ultrasound measures ejection fraction(EF)and left ventricular end-diastolic diameter(LVEDD);enzyme-linked immunosorbent assay to determine serum NT-proBNP,CRP and TNF-αconcentration;electrophysiological methods to detect heart rate,ERP;take myocardial tissue for Masson staining was used to observe the morphological changes of myocardium.Results After 8 weeks of percutaneous ear vagus nerve stimulation treatment,the EF[(53.50±2.59)%]in the AB-VNS group was higher than the heart failure group[(45.17±3.06)%],and the LVEDD[(37.17±1.60)mm]was smaller than the heart failure group[(53.83±6.15)mm],the difference was statistically significant(P<0.05);AB-VNS group Nt-proBNP was lower than heart failure group[(388.56±40.94)pg/ml vs(674.91±56.78)pg/ml],CRP[(9.57±1.18)mg/L vs(9.57±1.18)mg/L],TNF-α[(6.40±0.63)ng/L vs(10.82±0.88)ng/L],resting heart rate[(124.17±7.30)bpm vs(146.67±8.14)bpm],ERP was longer than heart failure group[(158.50±5.24)ms vs(125.17±4.83)ms],the difference was statistically significant(P<0.05).Masson staining in AB-VNS group showed that the shape of myocardial cells was regular,with a small amount of collagen fibers.Conclusion Low-intensity percutaneous vagus nerve branch stimulation can help increase heart ejection fraction,reduce NT-proBNP and inflammatory factors,and slow heart rate and prolong the effective refractory period in dogs with heart failure.
作者
赵利
苏玮
王坤
郭玉君
ZHAO Li;SU Wei;WANG Kun;GUO Yu-jun(Department of Heart Failure,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang,China;The Second Affiliated Hospital of Guizhou Medical University,Kaili 556000,Guizhou,China)
出处
《医学信息》
2020年第11期58-60,71,共4页
Journal of Medical Information
基金
国家自然科学基金项目(编号:81660055)。
关键词
心力衰竭
迷走神经耳支
心功能
心衰犬
Heart failure
Vagus nerve ear branch
Heart function
Heart failure dog
