摘要
以沪农灵芝(Ganoderma lucidum)1号子实体为原料,经过87%乙醇提取、D101大孔树脂富集、乙醇梯度洗脱获得弱极性三萜组分。采用高速逆流色谱技术,以正己烷∶乙酸乙酯∶甲醇∶水(V∶V∶V∶V=5∶6∶5∶5)为溶剂体系,对该三萜组分中灵芝酮三醇的制备工艺进行研究,在转速900 r·min^-1、流速3 mL·min^-1条件下,一次上样400 mg灵芝弱极性三萜组分,可得到纯度为91.6%的灵芝酮三醇26.8 mg。
Ganodermanontriol was purified from fruiting bodies of Ganoderma lucidum(Hunong No.1) following a series of steps, including extraction by 87% ethanol, condensation by macroporous adsorption resin D101, gradient elution by ethanol to obtain the weak polar triterpenes fraction(WTF), and finally preparation of the target compound from WTF by high-speed countercurrent chromatography(HSCCC). For HSCCC, the solvent system was determined to be a mixture of N-hexane,ethyl acetate, methanol and water(V∶V∶V∶V=5∶6∶5∶5);the separation condition was set as rotation at 900 rpm and flow rate of 3.0 mL·min^-1. Using the above mentioned HSCCC condition, a single loading amount of 400 mg WTF yielded 26.8 mg ganodermanontriol with a purity of 91.6%.
作者
岳亚文
田振
张劲松
王晨光
韩伟
唐庆九
冯娜
周帅
YUE Yawen;TIAN Zhen;ZHANG Jingsong;WANG Chenguang;HAN Wei;TANG Qingjiu;FENG Na;ZHOU Shuai(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;Engineering Research Centre of Pharmaceutical Process Chemistry,Ministry of Education,East China University of Science and Technology,Shanghai 200237,China;Shanghai Baixin Bio-Tech Co.,Ltd.,Shanghai 201403,China;Shanghai Ocean University,Shanghai 201306,China)
出处
《食用菌学报》
CSCD
北大核心
2020年第2期77-83,共7页
Acta Edulis Fungi
基金
上海市科学技术委员会科研计划项目(17391903300)。
关键词
灵芝子实体
弱极性三萜
大孔树脂
高速逆流色谱
Ganoderma lucidum fruiting body
weak polar triterpenes
macroporous adsorption resin
high-speed countercurrent chromatography(HSCCC)
