摘要
目的:探讨口服蛋氨酸饲料致大鼠心力衰竭模型的机制,及程序性死亡因子4(PDCD4)通路在此过程中的作用。方法:5周龄健康Wistar雄性大鼠30只,适应性喂养1周后,随机分为实验组与对照组,实验组给予3%蛋氨酸饲料喂养,对照组标准饲料喂养。12周后进行血流动力学检测,采用酶联免疫法测定血清同型半胱氨酸(HCY)及N端前脑钠肽(NT-proBNP)水平,心脏彩超进行心功能评估,病理染色明确有无细胞肥大、纤维化,Western blot检测心肌组织中PDCD4蛋白的表达。结果:3%蛋氨酸饲料喂养组HCY水平较对照组升高(均P<0.05),心脏彩超提示3%蛋氨酸饲料喂养组大鼠左室射血分数、缩短分数、室间隔厚度显著减低(均P<0.05),左室收缩末内径及舒张末内径较对照组显著增加(均P<0.05),Western blot结果显示,PDCD4蛋白表达降低(P<0.05)。结论:通过口服蛋氨酸饲料造成大鼠高HCY血症可导致大鼠出现心室重塑,并造成心功能受损,其机制可能与PDCD4信号通路的下调有关。
Objective:To investigate the mechanism of methionine-induced heart dysfunction in rats and the role of programmed cell death factor(PDCD4)pathway in this process.Methods:Thirty healthy male Wistar rats were randomly divided into control group or 3%methionine forage fed group after adaptive feeding for 1 week.Twelve weeks later,the hemodynamic assessment were performed.The level of serum homocysteine(HCY)and N-terminal B-type natriuretic peptide(NT-proBNP)were determined by enzyme-linked immunosorbent assays.The cardiac function was evaluated by color Doppler echocardiography,and the ventricular changes were confirmed by pathological stains.Finally,the expression of PDCD4 in ventricle was detected by Western blot.Results:The level of HCY in the experimental group was higher than that in the control group(P<0.05).Echocardiographic showed the left ventricular ejection fraction,fractional shortening,interventricular septal thickness in the experimental rats were decreasing(all P<0.05),while the left ventricular end diastolic dimension and left ventricular end systolic dimension were increasing compared to the control rats(all P<0.05).Besides,the expression of protein PDCD4 was lower in experimental rats(P<0.05).Conclusion:High homocysteinemia induced by methionine feed can lead to myocardial dysfunction and ventricular remodeling in rats,and it may be related to the down-regulation of PDCD4 signaling pathway.
作者
涂代苗
阚晨星
马向红
TU Dai-miao;KAN Chen-xing;MA Xiang-hong(Department of Cardiology,The Second Hospital,Tianjin Medical University,Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease,Tianjin Institute of Cardiology,Tianjin 300211,China)
出处
《天津医科大学学报》
2020年第3期213-217,共5页
Journal of Tianjin Medical University
基金
天津医科大学第二医院青年基金项目资助(2017ydey18)。
