摘要
目的观察青叶胆对小鼠慢性肝损伤的保护作用及其对小鼠脾脏T淋巴细胞亚群的调节作用。方法采用腹腔注射四氯化碳(CCl_4)造成小鼠化学性肝损伤模型,检测小鼠血清天冬氨酸转氨酶(aspartateaminotransferase,AST)、丙氨酸转氨酶(alanine aminotransferase,ALT)、总蛋白(total protein,TP)及总胆红素(total bilirubin,TBI);HE染色,观察肝组织形态;流式细胞术检测CD3^+、CD4^+及CD8^+阳性细胞数的变化情况,计算CD4^+/CD8^+比例,并与模型组和对照组比较。结果青叶胆对CCl_4造成的小鼠慢性肝脏损伤具有保护作用,与模型组相比,能明显降低小鼠血清ALT、AST含量(P<0.01),升高TP含量(P<0.01);与模型组相比,青叶胆低剂量组对CD3^+细胞具有下调作用(P<0.05),对CD8^+细胞的上调作用和对CD4^+/CD8^+比值的下调作用具有显著性差异(P<0.01);高剂量组对CD8^+细胞的上调作用和对CD4^+/CD8^+比值的下调作用具有统计学意义(P<0.05)。结论青叶胆对CCl4诱导的小鼠慢性肝损伤具有保护作用,其机制可能是通过调节T淋巴细胞亚群,提高免疫功能。
OBJECTIVE To observe the protective effect of Swertia mileensis T.M.Host W.L.Shih on chronic liver injury in mice and its regulation on spleen T lymphocyte subsets in mice.METHODS A model of chemical liver injury in mice was induced by intraperitoneal injection of carbon tetrachloride(CCl4).Serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),total protein(TP)and total bilirubin(TBI)were detected in mice.HE staining was used to observe liver tissue morphology;flow cytometry was used to detect changes in the number of CD3+,CD4+and CD8+positive cells;the proportion of CD4^+/CD8^+ratio were calculated,and they were compare with the model group and the control group.RESULTS S.mileensis had protective effects on chronic liver injury induced by CCl4 in mice,which could significantly decrease serum ALT and AST levels(P<0.01)and increase TP content compared with model group(P<0.01).Compared with model group,the low dose group of S.mileensis had a down-regulating effect on CD3^+cells(P<0.05),and there was a significant difference in the up-regulating effect on CD8^+cells and the down-regulating effect on the ratio of CD4^+/CD8+(P<0.01);the high dose group of S.mileensis had a statistically significant effect on the up-regulation of CD8+cells and the down-regulation of CD4^+/CD8+ratio(P<0.05).CONCLUSION S.mileensis has protective effect on CCl4-induced chronic liver injury in mice,and its mechanism may be through the regulation of T lymphocyte subsets,affecting immune function.
作者
陶爱恩
赵飞亚
黎氏文梅
夏从龙
李水仙
TAO Ai’en;ZHAO Feiya;LISHI Wenmei;XIA Conglong;LI Shuixian(School of Medicine,Tourism and Culture College of Yunnan University,Lijiang 674199,China;Dali University,School of Pharmacy and Chemistry,Dali 671000,China;Dali University,School of Basic Medicine,Dali 671000,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2020年第8期935-938,共4页
Chinese Journal of Modern Applied Pharmacy
基金
云南省科技厅科技计划项目(2018FD072)
大理大学青年教师科研基金项目(KYQN201505)。
关键词
藏药
青叶胆
慢性肝损伤
T淋巴细胞亚群
免疫调节
Tibetan medicine
Swertia mileensis T.M.Host W.L.Shih
chronic liver injury
T lymphocyte subsets
immunomodulation
