摘要
以17年生元宝枫人工林为试验材料,研究叶面喷施激动素和2,4-表油菜素内酯对其叶绿素含量、叶片和翅果中糖含量(可溶性糖、蔗糖、淀粉)、蔗糖代谢酶(蔗糖磷酸合成酶、蔗糖合成酶分解方向、蔗糖合成酶合成方向)活性的影响,分析外源激素调控元宝枫叶片和翅果碳水化合物代谢的作用机理,为元宝枫的丰产稳产提供理论指导。结果表明,元宝枫叶面喷施激动素和2,4-表油菜素内酯可以提高叶绿素含量,增强光合作用,促进光合产物的积累,叶片中可溶性糖含量和蔗糖含量升高,蔗糖磷酸合成酶活性显著增强,叶片源强提高,促进光合产物向翅果运输;翅果中可溶性糖含量、蔗糖含量与淀粉含量均得到提高,蔗糖合成酶分解活性增强,蔗糖磷酸合成酶活性和蔗糖合成酶合成活性减弱,促进翅果中蔗糖的分解转化,有利于翅果的生长发育以及品质产量的提高。
Taking 17-year-old Acer truncatum stand as research object,effects of spraying exogenous kinetin and 2,4-epibrassinolide on carbohydrate accumulation and related enzyme activity of A.truncatum were analyzed to provide a theoretical basis for high and stable seed yield.The contents of chlorophyll,soluble sugar,sucrose and starch,activities of sucrose phosphate synthase and sucrose synthase in the leaves and samaras were measured.The results indicated that exogenous kinetin and 2,4-epibrassinolide led to significant increases in chlorophyll content,which increased photosynthesis and accelerated accumulation of photosynthetic products.Increased contents of soluble sugar and sucrose in leaf were observed with both kinetin and 2,4-epibrassinolide treatments.Leaf source strength increased after foliar spray compared to the control due to higher sucrose phosphate synthase activity.Soluble sugar,sucrose and starch contents of A.truncatum samara increased significantly after spraying kinetin and 2,4-epibrassinolide.Sucrose synthase in the direction of cleavage was higher under treatments compared with the control,whereas decreased activities of sucrose phosphate synthase and sucrose synthase were observed in the samara.These changes were largely contributed to the degradation and transformation of sucrose,development of samara,and improvement of quality and yield.
作者
郑豪亮
张晨晨
张博勇
王迪海
ZHENG Hao-liang;ZHANG Chen-chen;ZHANG Bo-yong;WANG Di-hai(College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)
出处
《西北林学院学报》
CSCD
北大核心
2020年第3期100-105,共6页
Journal of Northwest Forestry University
基金
国家重点研发计划课题(2017YFC0504605)
西北农林科技大学试验示范站科技成果推广项目(TGZX2019-3)
陕西省重点研发计划项目(2017ZDXM-N-23-10)。
关键词
元宝枫
激动素
2
4-表油菜素内酯
糖含量
蔗糖代谢酶
Acer truncatum
kinetin
2,4-epibrassinolide
sugar content
sucrose metabolism enzyme
