摘要
目的:探究水飞蓟宾对过氧化氢(H2O2)诱导SH-SY5Y细胞损伤的保护作用及可能机制。方法:采用体外培养方法,建立SH-SY5Y细胞的H2O2损伤模型。用水飞蓟宾预处理细胞后MTT法测定细胞的存活率;流式细胞术检测活性氧(reactive oxygen species,ROS)、细胞凋亡和线粒体膜电位的变化;Western blot法检测Cleaved-Caspase-9、Cleaved-Caspase-3蛋白表达情况。结果:与模型组比较,用50和100μmol/L水飞蓟宾预处理SH-SY5Y细胞3 h能明显减轻H2O2诱导的SH-SY5Y损伤,显著提高细胞存活率(P <0.01)和减少凋亡(P <0.05),减少细胞内ROS水平,稳定线粒体膜电位(P <0.05),使Cleaved-Caspase-9和Cleaved-Caspase-3的表达量下降。结论:水飞蓟宾可以保护SH-SY5Y细胞免受H2O2的损伤,其机制可能与水飞蓟宾的抗氧化性、保护线粒体膜电位和降低Cleaved-Caspase-9和Cleaved-Caspase-3的表达量有关。
Objective:To study the protective effect and possible mechanism of silibinin on cell damage of SH-SY5 Y induced by hydrogen peroxide(H2O2). Methods:SH-SY5 Y cell damage model was established by H2O2 in vitro. After pretreatment with silibinin,the cell viability was determined by MTT,reactive oxygen species(ROS),apoptosis and mitochondrial membrane potential(MMP)were determined by flow cytometry,and the expressions of Cleaved-Caspase-9 and Cleaved-Caspase-3 were detected by Western blot.Results:Compared to the model group,pretreatment with 50 μmol/L and 100 μmol/L silibinin for 3 h attenuated the damage of SHSY5 Y cells treated with H2O2,improved the cell survival rate(P<0.05),reduced the apoptosis rates(P<0.05)and the release of ROS,stabilized MMP(P<0.01),decreased the expression of Cleaved-Caspase-9 and Cleaved-Caspase-3. Conclusion:Silibinin can protect SH-SY5 Y cell from H2O2,which may be related to its antioxidant capacity,protection of mitochondrial membrane potential,and reduction of the expression of Cleaved-Caspase-9 and Cleaved-Caspase-3.
作者
荣家闵
于明
葛雅琨
张仁文
劳军
张元新
RONG Jiamin;YU Ming;GE Yakun;ZHANG Renwen;LAO Jun;ZHANG Yuanxin(College of Food&Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第4期515-520,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
吉林省省级产业创新专项资金(2018C049⁃4)
吉林省教育厅科研项目[吉教科合字(2016)第133号]
吉林市科技局项目(201750259)
吉林化工学院校级重大项目(20180101)。
