摘要
目的:探讨超极化激活环核苷酸门控阳离子通道(hyperpolarization-activated cyclic nucleotide-gated cation channels,HCN通道)4种亚型在大鼠脊髓背角浅层的表达与分布特点。方法:选取3~5周龄SD大鼠,雌雄不拘,制作L4~L5段脊髓横切片,应用免疫组织化学技术及激光共聚焦成像技术,观察HCN通道的4种亚型在脊髓背角浅层神经元、胶质细胞及神经元亚细胞结构中的分布。结果:HCN通道不同亚型在正常SD大鼠脊髓背角中的表达和分布具有特异性:(1)HCN1主要与神经元标志物[神经元核抗原(neuronal nuclei,NeuN)]和星形胶质细胞标志物[胶质细胞原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)]共存,HCN2和HCN3主要与NeuN共定位;(2)HCN2主要与肽能初级传入神经末梢标志物[降钙素基因相关肽(calcitonin gene-related peptide,CGRP)]共存,HCN4主要与非肽能初级传入神经末梢标志物[异凝集素B4(isolectin B4,IB4)]共存;(3)HCN1和HCN4主要与神经元树突标志物[微管相关蛋白2(microtubule-associated protein 2,MAP2)]共存;(4)HCN4主要与抑制性中间神经元轴突末梢标志物[囊泡γ-氨基丁酸转运体(vesicularγ-aminobutyric acid transporter,VGAT)]共存。结论:HCN1~4主要分布在大鼠脊髓背角浅层,且在神经元、胶质细胞及神经元亚细胞结构中呈特异性分布。
AIM:To investigate the distinct expression and localization of the 4 subtypes of hyperpolarizationactivated cyclic nucleotide-gated cation(HCN)channels in rat superficial spinal dorsal horn(SDH).METHODS:Transverse slices of L4~L5 segments were obtained from Sprague-Dawley(SD)rats(3~5-week-old)of either sex. Using the immunohistochemical technique and laser confocal imaging,the distribution of HCN1~4 in neurons,glia cells and subcellular structure of neuron in SDH was observed.RESULTS:The expression of 4 subtypes of HCN channel in rat SDH was distinct:HCN1 was mainly co-localized with neuronal marker neuronal nuclei(NeuN)and astrocyte marker glial fibrillary acidic protein(GFAP). HCN2 and HCN3 were largely co-localized with NeuN. HCN2 was primarily co-localized with peptidergic primary afferent nerve ending marker calcitonin gene-related peptide(CGRP). However,HCN4 was mainly co-localized with non-peptidergic primary afferent nerve ending marker isolectin B4(IB4). HCN1 and HCN4 were mainly co-localized with dendrite marker microtubule-associated protein 2(MAP2). HCN4 was primarily co-localized with axonal terminal of inhibitory interneuron marker vesicular γ-aminobutyric acid transporter(VGAT).CONCLUSION:HCN1~4 are predominantly distributed in superficial spinal dorsal horn and exhibit a specific expression pattern in neurons,glia cells and subcellular structure of neurons.
作者
黄姣艳
程小娥
马龙先
张达颖
蒋昌宇
柳涛
HUANG Jiao-yan;CHENG Xiao-e;MA Long-xian;ZHANG Da-ying;JIANG Changyu;LIU Tao(Department of Anesthesiology,The First Affiliated Hospi⁃tal of Nanchang University,Nanchang 330006,China;Department of Pain Clinic,The First Affiliated Hospi⁃tal of Nanchang University,Nanchang 330006,China;Center for Experimental Medicine,The First Affiliated Hospi⁃tal of Nanchang University,Nanchang 330006,China;Jisheng Han Academician Workstation for Pain Medicine,Nan⁃shan Hospital,Shenzhen 518052,China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2020年第5期827-836,共10页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81860216,No.31660289)。
关键词
超极化激活环核苷酸门控阳离子通道
脊髓背角
神经元
Hyperpolarization-activated cyclic nucleotide-gated cation channels
Spinal dorsal horn
Neurons
