摘要
目的研究赤芍及赤芍总单萜和赤芍总酚对热毒血瘀证大鼠的清热凉血散瘀功效,探讨赤芍清热凉血散瘀功效的物质基础。方法将SD大鼠随机分为空白组、模型组、清营汤组、赤芍组、赤芍总单萜组和赤芍总酚组,除空白组外各给药组连续灌胃相应药物(20 g/kg)预防性给药8 d,以腹腔注射角叉菜胶(25 mg/kg)联合尾静脉注射内毒素(80μg/kg)的方法复制大鼠热毒血瘀证动物模型,空白组灌胃等量的去离子水、注射生理盐水以代替药物。采用ELISA法检测大鼠血清前列腺素E2(PGE2)、环磷酸腺苷(cAMP)、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白介素-1β(IL-1β)、白介素-6(IL-6)、内皮素-1(ET-1)、一氧化氮(NO)、血栓素(TXB2)和6酮前列腺素F1α(6-keto-PGF1α)含量。采用发色底物法检测凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)和纤维蛋白原(FIB)值变化。结果与模型组比较,赤芍组及赤芍总酚组PGE2、cAMP、TNF-α、IFN-γ、IL-1β、IL-6含量降低(P<0.05),赤芍总单萜组cAMP、IFN-γ、IL-1β、IL-6含量降低(P<0.05);赤芍组及赤芍总单萜组TT、PT、APTT值升高(P<0.05),FIB值下降(P<0.05),NO、6-keto-PGF1α含量升高,NO/ET-1比值升高,ET-1、TXB2含量降低,TXB2/6-keto-PGF1α比值降低(P<0.05),赤芍总酚组NO含量和NO/ET-1比值均升高,ET-1和TXB2含量及TXB2/6-keto-PGF1α比值均降低(P<0.05)。结论赤芍总单萜和赤芍总酚对热毒血瘀证大鼠均有清热凉血散瘀的作用,但赤芍总酚清热凉血作用优于赤芍总单萜,赤芍总单萜的散瘀作用优于赤芍总酚。
Objective To investigate the effects of Chishao(Red Peony Root,Radix Paeoniae Rubra),its total monoterpenes and its total phenols on clearing heat,cooling blood and dissolving stasis in rats with heat toxicity and blood stasis pattern to discuss the material basis for such effects.Methods SD rats were randomly divided into the blank group,model group,Qingying Tang(Nutrient Aspect Heat-Clearing Decoction,QYT)group,Chishao group,Chishao total monoterpenes(CTM)group and Chishao total phenols(CTP)group.Models with heat toxicity and blood stasis pattern were established by intraabdominal injection of carrageenan(25 mg/kg)combined with tail vein injection of endotoxin(80μg/kg).At the same time,corresponding medicinals were administered to the treatment groups by gavage at 20 g/kg for 8 days consecutively.The same amount of deionized water was given by gavage and normal saline was injected to the blank group.The levels of PGE2,cAMP,TNF-α,IFN-γ,IL-1β,IL-6,ET-1,NO,TXB2 and 6-keto-PGF1αwere measured with ELISA.The changes in the levels of TT,PT,APTT and FIB were detected with chromogenic substrate method.Results Compared with model group,levels of PGE2,cAMP,TNF-α,IFN-γ,IL-1βand IL-6 were reduced in Chishao group and CTP group(P<0.05),and levels of cAMP,IFN-γ,IL-1βand IL-6 were lowered in CTM group(P<0.05).In Chishao group and CTM group,TT,PT and APTT levels were increased(P<0.05),FIB levels decreased(P<0.05),NO and 6-keto-PGF1αlevels and NO/ET-1 ratios increased(P<0.05),and ET-1 and TXB2 levels and TXB2/6-keto-PGF1αratio lowered(P<0.05).In CTP group,the contents of NO and the NO/ET-1 ratios were increased,and the levels of ET-1 and TXB2 and the TXB2/6-keto-PGF1αratios decreased(P<0.05).Conclusion Both CTM and CTP have heat-clearing,blood-cooling and stasis-dissolving effects on rats with heat toxicity and blood stasis pattern.However,CTP demonstrates better effect of clearing heat and cooling blood than CTM while the latter has better effect of dissolving stasis than the former.
作者
张建军
李伟
乐娜
王玉杰
周雪
张睿
Zhang Jianjun;Li Wei;Yue Na;Wang Yujie;Zhou Xue;Zhang Rui(School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2020年第3期196-202,共7页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.81473370)。
关键词
赤芍
赤芍总单萜
赤芍总酚
热毒血瘀证
清热凉血散瘀
大鼠
Chishao
total monoterpenes of Chishao
total phenols of Chishao
heat-toxin and bloodstasis pattern
clearing heat
cooling blood and dissolving stasis
rats
