摘要
目的比较血循环肿瘤细胞(CTCs)、循环游离DNA(cf DNA)联合检测与传统肿瘤标志物糖蛋白抗原153(CA153)检测在辅助诊断乳腺癌中的应用价值。方法回顾性选择2017年1月到2019年6月在安徽省第二人民医院收治的82例被确诊的乳腺癌患者为乳腺癌组,选取同期收治的50例乳腺良性疾病患者为乳腺良性疾病组,50例于该院体检的健康女性为对照组。采用ROCHE化学发光仪检测血清CA153水平;采用qPCR检测血浆cf DNA基因水平(241 bp hTERT和100 bp hTERT)和完整性(241 bp hTERT/100 bp hTERT);采用Ce1l Search系统检测血CTCs;受试者工作曲线(ROC)分析血CA153、cf DNA与CTCs诊断乳腺癌的诊断价值。结果乳腺癌组患者的血CTCs阳性表达率为37. 80%,明显高于乳腺良性疾病组的16. 00%(P <0. 05),明显高于对照组的12. 00%(P <0. 05);乳腺癌组的血CA153、241 bp hTERT、100 bp hTERT、241 bp hTERT/100 bp hTERT水平均明显高于乳腺良性疾病组(P <0. 05),乳腺良性疾病组的血CA153、241 bp hTERT、100 bp hTERT、241 bp hTERT/100 bp h TERT水平均明显高于对照组(P <0. 05);ROC分析显示,血cf DNA 241 bp hTERT、100 bp hTERT、241 bp hTERT/100 bp hTERT以及三者联合诊断乳腺癌的曲线下面积(AUC)依次为0. 865、0. 725、0. 870和0. 950;血CTCs和CA153诊断乳腺癌的AUC为0. 609和0. 733,明显低于cf DNA诊断的0. 950。结论血cf DNA对乳腺癌具有较高的诊断价值,有望应用于乳腺癌患者的临床辅助诊断。
Objective To compare application value of combined detection of plasma circulating tumor cells( CTCs) with circulating free DNA( cf DNA) and traditional tumor marker carbohydrate antigen 153( CA153) in adjuvant diagnosis of breast cancer. Methods Eighty-two patients who were confirmed with breast cancer and admitted to the hospital from January 2017 to June 2019 were enrolled in this study. 50 patients with benign breast disease who were admitted in the same period were selected as benign breast disease group. 50 healthy women who underwent physical examination in the hospital were enrolled as control group. The level of serum CA153 was detected by ROCHE chemiluminescence instrument. The levels of plasma cf DNA genes( 241 bp h TERT and 100 bp h TERT),and integrity( 241 bp h TERT/100 bp h TERT) were detected by q PCR. Plasma CTCs were detected by Ce1 l Search system. ROC analysis was performed to analyze diagnostic value of serum CA153,cf DNA and CTCs for breast cancer. Results The positive expression rate of plasma CTCs in breast cancer group was significantly higher than that in benign breast disease group( 37. 80% vs. 16. 00%,P < 0. 05),which was significantly higher than that in control group( 12. 00%)( P < 0. 05). The levels of serum CA153,241 bp h TERT,100 bp h TERT,241 bp h TERT/100 bp h TERT in breast cancer disease group were significantly higher than those in benign breast disease group( P < 0. 05). And the above indexes in breast cancer disease group were significantly higher than those in control group( P < 0. 05). ROC analysis showed that AUC of plasma cf DNA 241 bp h TERT,100 bp h TERT,241 bp h TERT/100 bp h TERT and their combination for diagnosis of breast cancer were 0. 865,0. 725,0. 870 and 0. 950,respectively. AUC of plasma CTCs and serum CA153 for diagnosis of breast cancer were 0. 609 and 0. 733,which was significantly lower than that of cf DNA( 0. 950). Conclusion Plasma cf DNA is of relatively higher diagnostic value for breast cancer,which is expected to be applied in clinically adjuvant di
作者
程朝晖
徐伟明
陈玉珠
CHENG Zhao-hui;XU Wei-ming;CHEN Yu-zhu(Department of Clinical Laboratory,The Second People's Hospital of Anhui,Hefei Anhui 230041,China)
出处
《临床和实验医学杂志》
2020年第9期994-997,共4页
Journal of Clinical and Experimental Medicine
基金
安徽省科技厅公共服务平台项目(编号:PT20081011)。
