摘要
以Lyofast LH 13瑞士乳杆菌和Lyofast LB 8保加利亚乳杆菌为原料菌,研究复合菌体富硒条件。在单因素试验基础上,利用响应面得出,最优富硒条件为:Lyofast LH 13瑞士乳杆菌和Lyofast LB 8保加利亚乳杆菌接种复合比例1∶1,加硒质量浓度10μg/mL,接种量5%,复合菌体培养后25 h后加入硒溶液,培养时间60 h。在该最优条件下,复合菌体含硒量554μg/g。硒转化率为55.4%。利用富硒的复合菌种发酵剂发酵的酸乳在贮藏期内有机硒含量达27.7μg/kg,具有较高的硒含量,且在贮藏期中,各项理化指标均处于较优水平。
The condition of selenium-riched of complex bacteria was studied using Lyofast LH 13 Lactobacillus helveticus and Lyofast LB 8 Lactobacillus bulgaricus as raw materials.Based on the single factor experiment,the response surface was used to determine the optimal condition for selenium-riched.The Lactobacillus helveticus and Lactobacillus bulgaricus were inoculated at a ratio of 1∶1,the concentration of selenium of 10μg/mL,the inoculum size of 5%selenium solution was added 25 h after culture,and the culture time was 60 h.Under the optimal condition,the amount of selenium-riched in mixed bacteria was 554μg/g.The conversion rate of selenium was 55.4%.The selenium content of fermented yogurt fermented by selenium-riched mixed bacteria reached 27.7μg/kg with high selenium content.During the storage period,the physical and chemical indexes of the fermented yoghurt were all at the optimal level.
作者
宋玉卿
汪鸿
耿浩源
吴楠
于殿宇
王俊国
SONG Yuqing;WANG Hong;GENG Haoyuan;WU Nan;YU Dianyu;WANG Junguo(Jilin Business and Technology College,Changchun 130507;College of Food Science and Technology,Northeast Agricultural University,Harbin 150030)
出处
《食品工业》
CAS
北大核心
2020年第3期25-30,共6页
The Food Industry
基金
黑龙江省教育厅研发项目大豆生物加工关键技术开发及示范(TSTAU-C2018011)
近红外光谱检测油脂中TFAs含量研究(STTAU-R2018010)。
关键词
接种
瑞士乳杆菌
保加利亚乳杆菌
富硒
酸牛乳
inoculation
Lactobacillus helveticus
Lactobacillus bulgaricus
selenium-riched
yogurt
