摘要
目的:评价干姜及其炮制品色差值与活性成分含量的相关性。方法:采用高效液相色谱法测定6种活性成分的含量,采用色彩色差计测定干姜及其炮制品的色差值[明度(L^*)、红绿色轴分量(a^*)、黄蓝色轴分量(b^*)]。采用SPSS 24.0软件对色差值与活性成分含量进行相关性分析。结果:姜酮、6-姜酚、8-姜酚、6-姜烯酚、二乙酰氧基-6-姜二醇和10-姜酚检测质量浓度的线性范围分别为2.65~105.90、10.15~406.00、4.87~194.80、5.28~211.20、6.14~245.70、7.02~280.80μg/mL(r均大于0.999);定量限分别为7.46、13.68、14.37、16.62、17.03、17.99 ng,检测限分别为2.24、4.11、4.31、4.99、5.11、5.40 ng;精密度、稳定性、重复性试验的RSD均小于3%;平均加样回收率分别为101.34%、102.14%、101.22%,103.12%、103.74%、103.54%,103.06%、98.55%、99.43%,99.36%、103.51%、101.21%,100.85%、99.42%、99.60%,100.39%、97.69%、103.84%(RSD均小于3%,n=3);含量分别为0~0.66、0.06~7.57、0.03~1.45、0.29~3.47、0.15~2.85、0.04~2.83 mg/g。色差值L^*、b^*与干姜不同炮制程度呈极显著负相关(P<0.01),a^*与炮制程度呈正相关(P<0.05);干姜炮制前后L^*、b^*与姜酮含量呈极显著负相关,与其余5种成分呈极显著正相关(P<0.01);a^*与姜酮呈极显著正相关(P<0.01),与其余5种成分无相关性(P>0.05);干姜及其炮制品与姜酮含量呈极显著正相关,与其余5种成分含量呈极显著负相关(P<0.01)。结论:干姜及其炮制品的色差值与其活性成分含量相关,即随炮制程度的加重,其a^*增加,L^*、b^*降低;姜酮含量升高,6-姜酚、8-姜酚、6-姜烯酚、二乙酰氧基-6-姜二醇、10-姜酚含量降低。
OBJECTIVE:To evaluate the correlation between color difference values and active constituent contents of crude and processed Zingiber officinale.METHODS:HPLC method was adopted to determint the content of 6 active constituents.The color difference values of crude and processed Z.officinale[lightness(L^*),red-green axis component(a^*),yellow-blue axis component(b^*)]were determined by chromatic aberration meter.SPSS 24.0 software was adopted for the correlation analysis between color difference values and active constituent contents.RESULTS:The linear range of zingiberone,6-gingerol,8-gingerol,6-shogaol,diacetoxy-6-gingerol and 10-gingerol were 2.65-105.90,10.15-406.00,4.87-194.80,5.28-211.20,6.14-245.70,7.02-280.80μg/m L(r>0.999).The limits of quantification were 7.46,13.68,14.37,16.62,17.03,17.99 ng,and the limits of detection were 2.24,4.11,4.31,4.99,5.11,5.40 ng,respectively.RSDs of precision,stability,and repeatability tests were all lower than 3%.The average recovery rates were 101.34%,102.14%,101.22%;103.12%,103.74%,103.54%;103.06%,98.55%,99.43%;99.36%,103.51%,101.21%;100.85%,99.42%,99.60%;100.39%,97.69%,103.84%(RSD were all lower than 3%,n=3),respectively.The contents of them were 0-0.66,0.06-7.57,0.03-1.45,0.29-3.47,0.15-2.85,0.04-2.83 mg/g,respectively.L^*and b^*values were negative correlated with the processing degree of Z.officinale significantly(P<0.01),a^*showed a significantly positive correlation with the processing degree(P<0.05).L^*and b^*values showed a significantly negative correlation with the content of zingiberone before and after processing,but positively correlated with the other five components(P<0.01).a^*showed a significantly positive correlation with the content of zingiberone,but had no correlation with other five components(P>0.05).The crude and processed Z.officinale were positive correlated with the content of zingiberone,negatively correlated with other five components(P<0.01).CONCLUSIONS:There is a certain correlation between the color difference values of crude and processed
作者
林华坚
张梓豪
孟江
王淑美
LIN Huajian;ZHANG Zihao;MENG Jiang;WANG Shumei(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University/Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica,State Administration of Traditional Chinese Medicine/Engineering Technology Research Center for Chinese Materia Medica Quality of Guangdong Universities,Guangzhou 510006,China)
出处
《中国药房》
CAS
北大核心
2020年第10期1197-1202,共6页
China Pharmacy
基金
国家自然科学基金资助项目(No.81873006,No.8110-2809)
国家中医药管理局第6批全国老中医药专家学术经验继承工作项目(No.国中医药人教发〔2017〕29号)
广东省教育厅重点平台和科研项目(No.2018KZDXM040,No.2016KTSCX064)。
关键词
干姜
炮制品
高效液相色谱法
色彩色差计
活性成分
含量
色差值
相关性分析
Crude Zingiber officinale
Processed product
HPLC
Chromatric aberration meter
Active constituent
Content
Color difference values
Correlation analysis
