摘要
以微波及亚硝基乙基脲为诱变剂,对黑曲霉出发菌株ZYC-06进行复合诱变育种。ZYC-06经微波照射40s、0.06mg/mL亚硝基乙基脲处理10min,最终选育出1株性能稳定且高产果胶酶的突变菌株ZYC-B03,其果胶酶活力为2790U/g,是出发菌株的2.88倍。
The complex mutation breeding of Aspergillus niger ZYC-06 is carried out by using microwave and nitrosoethylurea as the mutagens.ZYC-06 is treated with microwave irradiation for40 sand 0.06 mg/mL nitrosoethylurea for 10 min,and a mutant strain ZYC-B03 with stable performance and high-yield pectinase is selected.Its pectinase activity is 2790 U/g,which is 2.88 times of the original strain.
作者
杜国军
张以超
彭凯
田英华
刘祥
王松
DU Guo-jun;ZHANG Yi-chao;PENG Kai;TIAN Ying-hua;LIU Xiang;WANG Song(Qiqihar University,Qiqihar 161006,China;Key Laboratory of Agricultural Products Processing in Regular Institutions of Higher Learning in Heilongjiang Province,Qiqihar University,Qiqihar 161006,China)
出处
《中国调味品》
CAS
北大核心
2020年第5期75-77,82,共4页
China Condiment
基金
黑龙江省省属高等学校基本科研业务费科研项目(135309458)
黑龙江省植物性食品加工技术特色学科专项(YSTSXK201828)。
关键词
微波
亚硝基乙基脲
黑曲霉
诱变
果胶酶
microwave
nitrosoethylurea
Aspergillus niger
mutation
pectinase
