摘要
目的:研究中药头风颗粒对偏头痛模型脑膜组织形态的影响及对中脑导水管周围灰质c-fos、c-jun蛋白表达及mRNA表达的影响。方法:将120只SD大鼠随机分为正常对照组、模型组、头风颗粒治疗组、头风颗粒预防组,除正常对照组外;其余三组大鼠注射硝酸甘油(GTN),建立实验性偏头痛模型。造模30min后,模型组灌胃蒸馏水,治疗组灌胃头风颗粒,预防组于造模前灌胃头风颗粒1周。造模4h后,采集中脑导水管周围灰质(PAG)标本,通过苏木素-伊红(HE)染色方法,镜下观察各组大鼠脑膜组织形态,每组中随机取18只大鼠,通过实时荧光定量PCR(RT-PCR)方法,检测c-fos、c-jun mRNA表达,其余12只大鼠行免疫组化SABC法,检测c-fos、c-jun蛋白表达的阳性细胞数及灰度值。结果:与正常对照组比较,模型组大鼠脑膜有明显的毛细血管扩张、大量炎性细胞渗出等现象,头风颗粒治疗组和预防组上述现象减轻。与正常对照组相比,偏头痛模型组c-fos和c-jun mRNA表达增强、c-fos和c-jun阳性细胞数明显增多,差异有显著统计学意义(P<0.01);与偏头痛模型组比较,头风颗粒治疗组和头风颗粒预防组c-fos和c-jun mRNA表达明显减弱、c-fos和c-jun阳性细胞数明显减少,差异有显著统计学意义(P<0.01)。结论:头风颗粒可以减轻硝酸甘油引起的神经性炎症、抑制c-fos、c-jun mRNA表达及蛋白表达,影响中脑导水管周围灰质(PAG)痛觉信息的调控,缓解偏头痛症状。
Objective:By observing the effects of Toufeng granule on meningeal morphology and the expression of c-fos and c-jun in periaqueductal gray(PAG)of migraine rats to discuss the therapeutic mechanism of Toufeng granule.Methods:120 healthy SD rats were divided into 4 groups:normal group,model group,Toufeng granule treatment group and Toufeng granule prevention group.The nitroglycerin migrainous animal model was constructed with the nitroglycerin(NTG)subcutaneous injection.And the rats in the normal control group were injected with physiologic saline.30 min after intragastric administration,the rats in the model group were gavaged with distilled water,the treatment group rats were intragastric administration of Toufeng granules.The prevention group rats were intragastric administration of Toufeng granules for one week before model establishment.After NTG subcutaneous injection the specimen at 4 hours was obtained.To observe the meningeal morphology in rats with hematoxylin and eosin(HE)staining,to detect the expression of c-fos mRNA and c-jun mRNA by RT-PCR method,and detect the expression of c-fos and-cjun with the immunohistochemical method.Results:Compared with the normal control group,model group rats had obvious meningeal telangiectasia,intercellular loose structure,a large number of inflammatory cells exudation phenomenon,and the above phenomenon was decreased in Toufeng granule treatment group and Toufeng granule prevention group.The expression of c-fos and c-jun in PAG were increased in the model group compared with that in the normal group,the difference was statistically significant(P<0.01).The expressions of cfos and c-jun in PAG were decreased in Toufeng granule treatment group and Toufeng granule prevention group compared with that in the model group(P<0.01).Conclusion:Toufeng granule can reduce the neurogenic inflammation induced by nitroglycerin,reduce the expression of c-fos and c-jun,inhibit the transmission of nociceptive information in PAG.Toufeng granule could prevent and treat migraine by the abov
作者
于明秀
吕文学
署文杰
孙西庆
Yu Mingxiu;Lv Wenxue;Shu Wenjie;Sun Xiqing(Affiliated Hospital of Shandong University of TCM,Jinan 250014,China)
出处
《亚太传统医药》
2020年第3期24-28,共5页
Asia-Pacific Traditional Medicine
基金
山东省自然科学基金专项(ZR2011HL041)。
