摘要
研究旨在探求复合酶脱毛的最优工艺条件,并为牛皮的开发利用提供了一定的理论基础。本试验以牛皮酶法脱毛过程中加酶方式、酶活力配比、酶液浓度、酶解时间、酶解pH、酶解温度等为研究因素,在单因素实验基础上进行复合酶脱毛方法,结合响应面法对复合酶脱毛工艺进行优化。结果表明,碱性蛋白酶和中性蛋白酶采用先后加入的酶解方式,得到了碱性蛋白酶和中性蛋白酶的最适酶活力配比、酶解温度、酶液浓度、酶解时间、pH值,测得综合评分为0.756。响应面法分析结果表明,酶解温度和酶液浓度的交互作用对脱毛效果的影响显著,而其他因素交互作用不显著。
The optimum technological conditions for unhairing with compound enzyme was studied,and it provided theoretical foundation for development and utilization of cowhide.In the unhairing process with cowhide enzyme,the enzyme addition,enzyme activity ratio,enzyme concentration,enzymolysis time,enzymolysis pH and enzymolysis temperature were taken as research factors,and unhairing method with compound enzyme was conducted on the basis of single factor experiment,and response surface method was combined,and unhairing technology with compound enzyme was optimized.The results showed that the enzymolysis method that alkaline protease and neutral protease were added successively was adopted,the optimum enzyme activity ratio,enzymolysis temperature,enzyme concentration,enzymolysis time and pH value of alkaline protease and neutral protease were obtained,and the comprehensive score was 0.756.The results of response surface method showed that the interaction between enzymatic hydrolysis temperature and enzyme concentration had a significant effect on the unhairing effect,while the interaction between other factors was not significant.
作者
甜甜
李敬
雷元华
李天婵
谢鹏
张新军
杨建军
孙宝忠
张松山
TIAN Tian;LI Jing;LEI Yuanhua;LI Tianchan;XIE Peng;ZHANG Xinjun;YANG Jianjun;SUN Baozhong;ZHANG Songshan
出处
《肉类工业》
2020年第4期30-39,共10页
Meat Industry
基金
青藏高原传统肉制品品质提升技术示范2018YFD0502404-3
国家肉牛牦牛产业技术体系(CARS-37)
现代农业产业技术体系河北省肉牛产业创新团队(HBCT2018130204)。
关键词
牛皮
酶法脱毛
酶活力配比
响应面法
cowhide
unhairing with enzyme
enzyme activity ratio
response surface method
