摘要
从湖北省利川市齐岳山(海拔1 500~1 600 m)采集藤茶(Ampelopsis grossedentata)样品,采用60%乙醇超声提取总黄酮,利用紫外可见光分光光度计双波长(λ=208.4 nm,λ=293.2 nm)检测藤茶幼嫩茎尖总黄酮,平均含量高达453.67±11.26 mg/g;利用硅胶柱色谱及半制备高效液相分离到3个化合物,利用LC-HR-ESIMS、1D和2D NMR波谱数据进行分析,鉴定其结构分别为二氢杨梅素(1)、杨梅素(2)和花旗松素(3);通过高效液相(HPLC)检测分析发现,利川市藤茶样品中二氢杨梅素、杨梅素和花旗松素的含量为别186.31±10.43、13.83±1.32、14.76±2.26 mg/g。
The total flavonoids were extracted by 60% ethanol-ultrasound from the samples of Ampelopsis grossedentata collected from Qiyueshan mountain(1 500~1 600 m),Lichuan city, Hubei province. The total flavonoids in the young tips of Ampelopsis grossedentata was up to 453.67±11.26 mg/g detected by ultraviolet-visible spectrophotometer at the double-wavelength(λ=208.4 nm,λ=293.2 nm). Three compounds were purified from the organic extract using LC-HR-ESIMS,1D and 2D NMR spectral data analysis. Their structures were identified to be dihydromyricetin(1), myricetin(2),and taxifolin(3). The contents of dihydromyricetin, myricetin and taxifolin were 186.31 ±10.43, 13.83 ±1.32 and 14.76 ±2.26 mg/g respectively detected by HPLC.
作者
李宇
刘翠君
艾伦强
张宇
卢新鹏
何美军
冉亚兰
LI Yu;LIU Cui-jun;AI Lun-qiang;ZHANG Yu;LU Xin-peng;HE Mei-jun;RAN Ya-lan(Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences,Enshi 445000,Hubei,China;Medical School,Hubei Minzu University,Enshi 445000,Hubei,China;Agricultural and Rural Bureau of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,Hubei,China)
出处
《湖北农业科学》
2020年第4期98-101,共4页
Hubei Agricultural Sciences
基金
湖北省恩施州科技计划专项(XYJ2016000305)。
