摘要
目的探讨不同产地前胡划分规格等级后药材间质量特征差异,为前胡药材规格等级标准制订提供参考依据。方法于2018年1月份到宁国中溪、宁国南山、宁国云梯、安徽池州、浙江驮岭等5个产地采集已经产区分级的样品20批。分别选取10个个体测量选货条胡近根头部1/3处直径及全体长度;采用薄层色谱法,以白花前胡甲素、白花前胡乙素、白花前胡素E为对照品,对药材进行定性鉴别;按照2015年版《中华人民共和国药典》要求检测醇溶性浸出物;采用高效液相色谱法对前胡药材进行3种香豆素类成分含量的测定。结果同一产地不同等级间药材性状数据有交叉现象,但不同产地同一等级之间药材均值差异不大,在小范围内波动;20批样品在与对照品相同位置上均显相同颜色斑点;不同等级间药材浸出物含量均符合2015年版《中华人民共和国药典》要求,有一定规律性;不同等级间药材香豆素类成分含量变化存在一定差异。结论 5个产地药材质量较为一致;经产区划分的前胡不同等级药材间外观性状与内在成分之间未见明显规律性,但质量存在一定差异,可进一步深入研究。
Objective To explore the differences in quality characteristics between the Radix Peucedani of the ancestral cultivars of different producing areas, and to provide a reference for the establishment of the standards of the classification of Peucedani Radix. Methods In January 2018, 20 batches of graded samples were collected from five production areas including Ningguo Zhongxi, Ningguo Nanshan, Ningguo Yunti, Anhui Chizhou, and Zhejiang Tuoling. A total of 10 individuals were selected to measure the diameter at 1/3 of the head and the total length of Tiaohu. The thin layer chromatography method was used to qualitatively identify the medicinal materials with the reference materials of Praeruptorin A, Praeruptorin B, qianhucoumarin E. The alcohol-soluble extracts were tested according to the requirements of the 2015 edition of the Chinese Pharmacopoeia;and the content of three coumarins was determined by high performance liquid chromatography. Results The data of different grades of medicinal materials from the same place of origin had crossover phenomenon, but the mean values of different grades of medicinal materials from different places of origin had little difference and fluctuated in a small range. The 20 batches of samples all showed the same color spots in the same position as the control. The contents of the extracts of different grades of crude drugs all meet the requirements of the 2015 edition of the Chinese Pharmacopoeia, with certain regularity. There were some differences in the content of coumarin among different grades. Conclusion The quality of medicinal materials from the five producing areas regions is consistent. There is no obvious regularity between the appearance and internal components of different grades of Radix Peucedani in the region, but there are some differences in quality, which can be further studied.
作者
李芸芸
潘雅楠
郭婷婷
张虹
刘守金
高广印
LI Yunyun;PAN Ya′nan;GUO Tingting;ZHANG Hong;LIU Shoujin;GAO Guangyin(College of Pharmacy,Anhui University of Traditional Chinese Medicine,Anhui Province,Hefei230000,China;Anhui Guangyintang Traditional Chinese Medicine Corporation Co.,Ltd,Anhui Province,Bozhou236800,China)
出处
《中国医药导报》
CAS
2020年第10期26-30,共5页
China Medical Herald
基金
国家中药标准化项目(ZYBZH-Y-AH-02)。
关键词
白花前胡
药材
规格等级
质量
Radix Peucedani
Chinese herbal medicine
Specification grade
Quality
