摘要
目的探讨血管紧张素转换酶(ACE)和血管紧张素原(AGT)基因表达、基因多态性与青海孕产妇妊娠高血压疾病的相关性。方法选择210例妊娠高血压患者(HDCP组)和220例正常孕妇(CK组),运用限制性内切酶片段长度多态性聚合酶链反应(PCR-RFLP)方法检测AGT M235T、ACE I/D基因多态性。结果CK组ACE基因DD、ID、Ⅱ所占比例分别为28.18%、47.73%、24.09%,HDCP组分别为33.81%、51.90%、14.29%(P<0.05,故两组的ACE基因分布有差异),HDCP组和对照组ACE I/D多态性等位基因I和D频率分布有差异(P<0.05),HDCP组D等位基因频率高于对照组(χ^2=5.188,P<0.05),ACE基因型分布符合Hardy-Weinberg遗传平衡(χ^2=0.423,df=2,查表χ^2界值表,P>0.05,达到遗传平衡);CK组AGT基因MM、MT、TT所占比例分别为24.09%、43.64%、32.27%,HDCP组分别为15.71%、42.86%、41.43%(P<0.05,故两组的AGT基因分布差异有统计学意义),HDCP组和对照组AGT M235T多态性等位基因M和T频率分布有差异性(P<0.05),HDCP组T等位基因频率高于对照组(χ^2=6.796,P<0.05),AGT基因型分布符合Hardy-Weinberg遗传平衡(χ^2=3.242,df=2,查表χ^2界值表,P>0.05,达到遗传平衡)。结论ACE I/D多态性和AGT M235T多态性与青海省汉族妊娠期高血压疾病有关,D等位基因和T等位基因可能是妊娠高血压疾病的易感基因。
Objective To investigate the relationship between angiotensin converting enzyme(ACE)and angiotensinogen(AGT)gene expression,gene polymorphism and pregnancy-induced hypertension in Qinghai.Methods A total of 210 pregnant hypertensive patients(HDCP group)and 220 normal pregnant women(CK group)were enrolled.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was used to detect AGT M235 T and ACE I/D gene polymorphism.Results The proportions of ACE gene DD,ID,andⅡin CK group were 28.15%,47.73%,and 24.09%,respectively.The HDCP group was 33.81%,51.90%,and 14.29%,respectively(P<0.05).The frequency distribution of ACE I/D polymorphic alleles I and D was different between HDCP group and CK group(P<0.05).D allele frequency was higher in HDCP group than in CK group(χ^2=5.188,P<0.05).ACE genotype distribution was accorded with Hardy-Weinberg genetic balance(χ^2=0.423,df=2,check tableχ^2 boundary value table,P>0.05,reach genetic balance).The proportions of MM,MT and TT in CK group was 24.09%,43.64%and 32.27%,respectively.The HDCP group was 15.71%,42.86%,and 41.43%respectively(P<0.05).HDCP group and CK group AGT M235 T polymorphic alleles M and T frequency distribution were different(P<0.05).HDCP group T allele frequency was higher than the CK group(χ2=6.796,P<0.05).AGT gene were genotype distribution accorded with Hardy-Weinberg genetic balance(χ^2=3.242,df=2,check tableχ^2 boundary value table,P>0.05,reaching genetic balance).Conclusion The ACE I/D polymorphism and AGT M235 T polymorphism are associated with hypertensive disorder of pregnancy in Han nationality in Qinghai Province.D allele and T allele may be susceptible genes of pregnancyinduced hypertension.
作者
王茹
庄文婷
王香林
李红荣
李长兴
孔德霞
李建华
WANG Ru;ZHUANG Wen-ting;WANG Xiang-lin;LI Hong-rong;LI Chang-xing;KONG De-xia;LI Jian-hua(Department of Human Anatomy,Basic Medicine Science,Medical College of Qinghai University,Xining 810001,China;Department of Radiology,Huadu District People's Hospital,Guangzhou 510800,China;Department of Surgical,Gansu Wuwei Tumor Hospital,Gansu Wuwei 733000,China;Department of Gynecology,the Second People's Hospital of Xining City,Xining 810001,China)
出处
《解剖学报》
CAS
CSCD
北大核心
2020年第2期294-299,共6页
Acta Anatomica Sinica
基金
青海省科技厅计划项目(2015-ZJ-746,2019-SF-134)。
关键词
青海
血管紧张素转换酶
血管紧张素原
妊娠高血压
基因多态性
限制性内切酶片段长度多态性聚合酶链反应
孕妇
Qinghai
Angiotensin-converting enzyme
Angiotensinogen
Pregnancy-induced hypertension
Gene polymorphism
Polymerase chain reaction-restriction fragment length polymorphism
Pregnant woman
