摘要
目的探讨血管紧张素转换酶(ACE)基因插入/缺失(I/D)位点多态性与自发性蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)的关系。方法 166例自发性SAH患者按照是否合并CVS分为CVS组72例和对照组94例,采用ELISA法检测患者血清ACE水平,采用聚合酶链反应-限制性片段长度多态性法检测ACE基因I/D位点多态性。结果 SAH后13 d内血清ACE水平无显著变化(均P>0.05)。CVS组患者入院时血清ACE基线水平高于对照组(t=2.136,P=0.048)。ACE组和对照组比较,两组ACE基因I/D位点基因型和等位基因分布频率差异均具有统计学意义(χ~2=7.086,P=0.029;χ~2=6.080,P=0.014)。多因素logistic回归法分析结果显示对于自发性SAH患者,携带ACE基因I/D位点D等位基因是发生CVS的危险因素(OR=1.473,95%CI=1.068~2.610,P=0.021)。结论对于自发性SAH患者,ACE基因I/D位点多态性可能与CVS发生相关。
Objective To investigate the association between angiotensin-converting enzyme(ACE)gene insertion/deletion(I/D)polymorphism and cerebral vasospasm(CVS)in patients with spontaneous subarachnoid hemorrhage(SAH).Methods A total of 166 patients with spontaneous SAH were divided into CVS group(72 cases)and control group(94 cases)depending on presence or absence of CVS.The serum ACE level of the patients was determined by ELISA,and the ACE gene I/D polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism.Results There was no significant change in serum ACE level within 13 days after SAH(all P>0.05).The baseline serum ACE level of the patients in the CVS group was higher than that in the control group(t=2.136,P=0.048).There were significant differences in genotype and allele distribution frequency of the ACE gene I/D site between the CVS group and the control group(χ^2=7.086,P=0.029;χ^2=6.080,P=0.014).A multivariate logistic regression analysis showed that carrying the D allele at the ACE gene I/D site was a risk factor for CVS in patients with spontaneous SAH(odds ratio[OR]=1.473,95%confidence interval[CI]:1.068-2.610,P=0.021).Conclusions The ACE gene I/D polymorphism may be correlated with the development of CVS in patients with spontaneous SAH.
作者
马刘佳
郑云锋
康平
胡国良
MA Liu-Jia;ZHENG Yun-Feng;KANG Ping;HU Guo-Liang(Department of Neurosurgery,Affiliated Hospital of Yan'an University,Yan'an,Shaanxi 716000,China)
出处
《国际神经病学神经外科学杂志》
2019年第5期528-531,共4页
Journal of International Neurology and Neurosurgery
基金
陕西省社会发展科技攻关项目(2016SF-306)
