摘要
目的 探讨内皮素-1（endothelin-1,ET-1）、白介素-1β（interleukin-1β,IL-1β）、白介素-6（interleukin-6,IL-6）在脑脊液（cerebrospinal fluid,CSF）及血清中的差异表达及其临床意义.方法 采用酶联免疫双抗体夹心法（ELISA）对54例急性颅脑损伤（acute craniocerebral injury,ACI）患者和19例健康对照者CSF和血清中ET-1,IL-1β和IL-6表达水平进行检测.结果 急性颅脑损伤患者在脑脊液中的ET-1（33.27±12.74 ng/L）和血清中ET-1（78.78±33.00 ng/L）的表达存在显著性差异（t=9.45,P=0.000）;IL-1β在脑脊液和血清中分别是7.04±3.47 ng/L和8.20±3.53 ng/L,两者相比差异有统计学意义（t=2.00,P=0.048）;IL-6在脑脊液和血清中分别是4.73±1.89 ng/L和4.16±1.50 ng/L,两者相比也存在显著差异（t=2.14,P=0.034）,且脑脊液高于血清.对照组中脑脊液和血清中的ET-1分别是15.67±7.72 ng/L和32.34±10.64 ng/L,两者相比差异有统计学意义（t=5.30,P=0.000）;IL-1β在脑脊液和血清中分别是2.57±1.10 ng/L和3.91±1.10 ng/L,两者相比差异有统计学意义（t=3.76,P=0.001）;而脑脊液和血清中的IL-6分别是1.90±0.78 ng/L和1.76±0.65 ng/L,两者相比也存在显著差异（t=3.40,P=0.003）.结论 无论是颅脑损伤患者还是对照组血清中ET-1和IL-1β浓度均高于脑脊液;而两组中脑脊液IL-6高于血清,提示IL-6的表达可能是更直接的颅脑损伤严重程度评估指标.
Objective To elucidate clinical significance of the varied concentration of cytokines in patients with acute cranioce-rebral injury and the concentration of endothelin-1 （ET-1） ,interleukin-1β （1L-1β） ,interleukin-6 （1L-6） in cerebrospinal fluid （CSF） and serum were investigated in 54 patients. Methods In this study,54 patients with acute cranioeerebral injury were investigated. Concentration of ET-1 ,IL-1β and IL-6 in CSF and serum in 54 patients with acute craniocerebral injury and 19 control groups were detected by ELISA. Results The concentration of ET-1 in sera （78. 78±33.00 ng/L） were significant-ly higher than that of in CSF （33.27±12.74 ng/L） in patients with acute craniocerebral injury （t=9.45 ,P=0. 000）. At the same time,the concentration of IL-1β （8. 20±3.53 ng/L） in sera was higher than that of in cerebrospinal fluid 7.04±3.47 ng/L （t = 2.00, P= 0. 048）. Moreover,the concentration of IL-6 in the CSF （4. 37 ± 1.89 ng/L） was higher than of in sera （4. 16±1.50 ng/L） in patients with acute craniocerebral injury （t= 2.14, P= 0. 034）. The concentration of ET-1 （32.34±10. 64 ng/L） and IL-1β （3.91 ± 1.10 ng/L） in sera of control groups were significantly higher than the concentration of ET-1 （15.67±7.72 ng/L） and IL-1β （2.57±1.10 ng/L） in cerebrospinal fluid. But the concentration of II.-6 in the CSF （1.90 ±0.78 ng/L） was higher than that of in sera （1.76±0.65 ng/L） in control groups （t=3.40, P=0. 003）. Conclusion The concentration of ET-1 and IL-1β were lower in CSF than that of in sera between patients with acute craniocerehral injury and control groups. However, the concentration of IL-6 in CSF was higher than that of in the sera,which indicates that IL-6 may be the direct cytokine in estimating severity of acute craniocerebral injury.
出处
《现代检验医学杂志》
CAS
2013年第5期27-29,共3页
Journal of Modern Laboratory Medicine
基金
南京军区医学课题资助项目（09MB122）.
