摘要
目的分析实时荧光定量聚合酶链反应技术对结核性脑膜炎的诊断价值。方法研究将该院2018年1月-2019年6月期间收治的70例结核性脑膜炎患者作为研究对象展开调查,对患者进行随机分组后采取不同的诊断方式,一组应用常规涂片检测方式,一组应用实时荧光定量聚合酶链反应技术,分别作为对照组和实验组,对比两组患者的检测结果及灵敏度、漏诊率和特异度。结果实验组患者检出阳性29例,阳性检出率为82.86%,其数据高于对照组,数据差异有统计学意义(χ^2=20.901,P<0.05);实验组检测的灵敏度为82.86%,漏诊率为17.14%(6例),不存在误诊情况,特异度为100.00%,其灵敏度高于对照组(χ^2=20.901,P<0.05)。结论实时荧光定量聚合酶链反应技术诊断结核性脑膜炎检出率、灵敏度和特异度均比较高,具有一定的指导意义,但仍存在一定的漏诊情况,建议临床中结合多种检查方式联合诊断。
Objective To analyze the diagnostic value of real-time fluorescence quantitative polymerase chain reaction(RTQPCR) in tuberculous meningitis. Methods 70 patients with tuberculous meningitis admitted to the hospital from January 2018 to June 2019 were investigated. The patients were randomLy grouped into different diagnostic methods.One group used routine smear detection methods, the other group used real-time fluorescence quantitative polymerase chain reaction technology as control. The sensitivity, missed diagnosis rate and specificity of the two groups were compared. Results 29 cases were positive in the experimental group, the positive rate was 82.86%. The data of the experimental group were higher than those of the control group, the difference was statistically significant(χ^2=20.901,P<0.05).The sensitivity of the experimental group was 82.86%, the missed diagnosis rate was 17.14%(6 cases). There was no misdiagnosis, and the specificity was 100.00%. The sensitivity of the experimental group was higher than that of the control group(χ^2=20.901,P<0.05). Conclusion The detection rate, sensitivity and specificity of real-time fluorescence quantitative polymerase chain reaction in the diagnosis of tuberculous meningitis are relatively high, which has certain guiding significance, but there are still some missed cases. It is suggested that clinical diagnosis should be combined with a variety of examinations.
作者
李佳
LI Jia(Department of Infectious Diseases,Honghe First People's Hospital,Mengzi,Yunnan Province,661199 China)
出处
《系统医学》
2020年第3期40-42,共3页
Systems Medicine
关键词
实时荧光定量聚合酶链反应技术
结核性脑膜炎
灵敏度
特异度
Real-time fluorescence quantitative polymerase chain reaction technology
Tuberculous meningitis
Sensitivity
Specificity
