摘要
【目的】了解喷施山梨醇及其类似物对桃叶片、果实中钾、钠、钙运输积累和对内源激素含量的影响。【方法】使用山梨醇及其结构类似的糖醇类渗透调节物(甘露醇、异山梨醇)喷施桃树体,测定桃叶、桃果实中的钠、钾、钙含量以及内源植物激素含量变化。基于测定结果,Q-PCR检测桃叶、桃幼果中相关离子转运子与特定激素合成通路关键限速酶基因的表达。【结果】喷施处理组中,果实钠、钾、钙含量显著增加(其中异山梨醇处理组中果实钠、钾、钙含量增加的幅度最大);但叶片中钠、钾、钙变化不显著。Q-PCR检测表明:钠离子转运相关的PpNHX7/SOS1在处理组叶片、果实中表达增强。钾离子转运相关的PpKUPs、PpKEAs在处理组叶片中表达趋势增强,在果实中PpKUPs表达增强、PpKEAs表达减弱。此外,喷施处理组中,桃果实内源性玉米素含量升高,玉米素合成通路关键限速酶基因PpIPT和PpCYP735A表达上调。【结论】山梨醇及其类似物喷施可能造成渗透胁迫,促使果实积累钠、钾、钙,并促进玉米素的合成以响应胁迫。
【Objective】Sorbitol,a polyol,is an important carbohydrate metabolite which plays important roles in photoassimilate translocation and carbohydrate storage in species of Rosaceae.In peach,a known sorbitol-synthetizing species,osmotic tolerance can be improved by synthesizing and accumulating polyols.Application of exogenous sorbitol has been shown to alter carbohydrate allocation in peaches.However,the metabolic changes induced by exogenous sorbitol and their physiological relevance in peach are still poorly understood.This study aims to investigate the effects of exogenous sorbitol and its analogs on iron transport and hormone contents in peach,and to uncover the underlying molecular and biochemical mechanisms.【Methods】’MX14-1’peach trees cultivated in an orchard located in Dahu town,Minhou county,Fuzhou,China,were chosen for the study.The peach trees were divided into four groups sprayed twice with 1 mmol·L-1 sorbitol,mannitol,isosorbide solution,or clean water(as control).After the 2 ndspray,leaves and fruit were harvested and transported in dry ice to the laboratory within one day.Sodium,potassium and calcium contents and endogenous phytohormones(ZA,ZR,GA,IAA and ABA)concentrations in peach leaves and fruit were determined.Based on the results,realtime RT-PCR was performed to quantify and verify the transcriptional changes of genes involved in ion transport and zeatin biosynthesis.【Results】Compared to the control group,the contents of sodium,potassium and calcium in the fruit increased significantly.The highest sodium,potassium and calcium contents were found in the fruit of isosorbide treatment group,being 53.89%,35.17%and 46.99%higher than in control fruit,respectively.In contrast to the accumulation of sodium,potassium and calcium in the fruit,no significant change in sodium,potassium,or calcium contents were observed in the leaves in various groups.Gene expression analyses using real-time RT-PCR showed that the spraying treatments had triggered an increase in PpNHX7/SOS1(aNHX-type Na+/H+an
作者
周平
马昕怡
郭瑞
颜少宾
张小丹
林志聪
金光
ZHOU Ping;MAXinyi;GUO Rui;YAN Shaobin;ZHANG Xiaodan;LIN Zhicong;JIN Guang(Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,Fujian,China;Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Department of Plant Biology,University of Illinois at Urbana-Champaign,Urbana,IL 61801,USA)
出处
《果树学报》
CAS
CSCD
北大核心
2020年第4期502-510,共9页
Journal of Fruit Science
基金
福建省省属公益类科研院所基本科研专项(2017R1013-1)
国家现代农业产业技术体系建设专项资金(CARS-30-Z-07)
福建省农业科学院创新团队(STIT2017-1-4)。
关键词
桃
山梨醇
离子
激素
基因表达
Peach
Sorbitol
Iron
Phytohormone
Gene expression
