摘要
通过根系施加脱落酸(ABA)合成抑制剂钨酸钠,研究盐胁迫(150 mmol·L-1 NaCl)下菊芋根系ABA信号对根系Na+转运、叶片Na+积累和光系统Ⅱ(PSⅡ)的影响。结果表明:钨酸钠抑制盐胁迫下根系ABA合成,降低根系Na+外排,提高根系Na+向叶片的转运系数。盐胁迫增加叶片Na+含量,没有影响叶片膜脂过氧化、PSⅡ反应中心蛋白合成和PSⅡ最大光化学效率(Fv/Fm)。根系ABA合成受抑制,显著增加盐胁迫下叶片Na+积累,加剧叶片膜脂过氧化,损伤PSⅡ反应中心蛋白,显著降低Fv/Fm,诱发PSⅡ光抑制。总之,盐胁迫下菊芋根系ABA信号诱导根系Na+外排,抑制Na+向地上部转运,有利于减少叶片Na+积累,防御PSⅡ氧化损伤。
The effects of root abscisic acid(ABA) signal on Na+ transport and photosystem Ⅱ(PSⅡ) in Jerusalem artichoke(Helianthus tuberosus) under salt stress(150 mmol·L-1 NaCl) were examined by applying ABA synthesis inhibitor sodium tungstate to roots. Sodium tungstate inhibited ABA synthesis in roots, reduced root Na+ efflux, and increased the efficiency of Na+ transport from roots to leaves under salt stress. Salt stress increased leaf Na+ content and did not affect leaf membrane lipid peroxidation, PSⅡ reaction center protein and PSⅡ maximum photochemical efficiency(Fv/Fm). The inhibition on root ABA synthesis significantly increased leaf Na+ accumulation, aggravated leaf membrane lipid peroxidation, impaired PSⅡ reaction center protein, decreased Fv/Fm, and induced PSⅡ photoinhibition. In conclusion, root ABA signal was beneficial to reducing leaf Na+ accumulation and preventing PSⅡ oxidative damage by inducing root Na+ efflux and inhibiting Na+ transport to the aerial part in H. tuberosus under salt stress.
作者
边甜甜
颜坤
韩广轩
安孟鑫
杨润亚
BIAN Tian-tian;YAN Kun;HAN Guang-xuan;AN Meng-xin;YANG Run-ya(School of Life Sciences,Ludong University,Yantai 264025,Shandong,China;CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences(CAS),Yantai 264003,Shandong,China;School of Agriculture,Ludong University,Yantai 264025,Shandong,China)
出处
《应用生态学报》
CAS
CSCD
北大核心
2020年第2期508-514,共7页
Chinese Journal of Applied Ecology
基金
中国科学院重点部署项目(KFZD-SW-112)
山东省自然科学基金项目(ZR2017QC005)资助~~
关键词
非损伤微测
光系统Ⅱ
钨酸钠
氧化胁迫
non-invasive micro-test
photosystem Ⅱ
sodium tungstate
oxidative stress
