摘要
目的基于网络药理学和生物信息学方法探究左金丸抗幽门螺杆菌(Hp)感染的分子网络调控机制。方法通过中药系统药理学数据库和分析平台(TCMSP)检索左金丸化学成分,筛选并预测其入血活性成分和作用靶点,检索疾病数据库中与Hp感染相关的作用靶点,构建左金丸成分靶点与疾病靶点的交互网络,获得左金丸抗Hp的特征性基因;通过DAVID6.8数据库对上述特征性基因进行GO功能富集和KEGG通路富集分析;利用Cytohubba筛选出左金丸抗Hp感染的关键靶点。结果通过TCMSP筛选、预测得到左金丸32个入血活性成分和197个作用靶点。GO分析共得到199条富集结果,其中生物过程包含炎症反应、RNA信号转录、信号传导等152条,细胞组分包含细胞核、细胞外基质、蛋白复合物等19条,分子功能包含细胞因子活性、DNA结合、ATP结合等28条。KEGG分析结果显示,Jak-STAT信号通路、T细胞受体信号通路、细胞周期信号通路、Wnt信号通路等65条通路与左金丸抗Hp感染密切相关。经Cytohubba筛选得到CXCL8、IL10、IL4、VEGFA、MMP9等10个关键基因。结论左金丸抗Hp感染具有多成分、多靶点、多通路协同作用的特点,可为其活性成分研究和抗Hp药效及机制研究提供依据。
Objective To study the molecular network regulation mechanism of Zuojin Pills against Helicobacter pylori(Hp) infection based on network pharmacology and bioinformatics. Methods By searching all the components of Zuojin Pills in TCMSP, the possible active components and targets were screened and predicted. The targets related to Hp infection were selected in 5 major disease databases, and then the interaction network between component targets and disease targets was constructed to obtain the characteristic gene of Zuojin Pills against Hp infection. GO functional enrichment and KEGG pathway enrichment analysis were performed on the above characteristic genes through DAVID6.8 database. Cytohubba was used to screen the key anti-Hp targets of Zuojin Pills. Results Totally 32 active components and 197 action targets of Zuojin Pills were screened and predicted by TCMSP. A total of 199 enrichment results were obtained by GO analysis, including 152 biological process of inflammatory response, RNA signal transcription and signal transduction, 19 cellular components of nucleus, extracellular matrix and protein complexes, and 28 molecular function of cytokine activity, DNA binding and ATP binding. KEGG analysis showed that 65 signaling pathways, such as Jak-STAT signaling pathway, T cell receptor signaling pathway, cell cycle signaling pathway and Wnt signaling pathway, were closely related to anti-Hp infection of Zuojin Pills. 10 key genes such as CXCL8, IL10, IL4, VEGFA and MMP9 were screened by Cytohubba. Conclusion Zuojin Pills have the characteristics of multi-components, multi-targets, and integral regulation for the treatment of Hp infection, which can provide basis for the study of active components and efficacy and mechanism of anti-Hp infection.
作者
陈新怡
曾梅艳
宋厚盼
陈小娟
林也
蔡雄
喻嵘
彭清华
CHEN Xinyi;ZENG Meiyan;SONG Houpan;CHEN Xiaojuan;LIN Ye;CAI Xiong;YU Rong;PENG Qinghua(Hunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,China;College of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2020年第5期90-96,共7页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81703920)
中国博士后科学基金(2019M662790)
湖南省自然科学基金(2019JJ50442)
湖南省中医药管理局科研一般项目(201780)
湖南省大学生研究性学习和创新性实验计划(2017280、201908)
湖南中医药大学校级科研基金(2018XJJJ28)
湖南中医药大学青年教师科研基金(201610)
湖南中医药大学中医学一流学科开放基金(2018ZYX20)。
关键词
左金丸
幽门螺杆菌
网络药理学
分子机制
信号通路
Zuojin Pills
Helicobacter pylori
network pharmacology
molecular mechanism
signaling pathway
