摘要
目的评估类风湿因子对血清甘胆酸测定的影响并探讨结果审核办法。方法用含有高水平类风湿因子的血清分别稀释不同水平的甘胆酸血清样本,测定原始和不同稀释度的血清样本类风湿因子和甘胆酸水平,观察稀释后测定结果的变化曲线,确认干扰存在;同时平行检测抗核抗体,观察干扰情况。结果添加类风湿因子后的血清甘胆酸测定结果下降,与类风湿因子水平呈正相关,差异有统计学意义(P<0.05)。类风湿因子水平在545 IU/mL以下时对血清甘胆酸测定基本没有影响,随着水平升高,干扰强度上升,甘胆酸检测结果急剧下降;未观察到抗核抗体干扰甘胆酸的测定。结论血清样本中较高水平的类风湿因子会干扰甘胆酸的测定,导致血清甘胆酸水平假性降低,需要引起临床重视。结果审核时应结合血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、总胆汁酸综合分析报告血清甘胆酸结果。
Objective To evaluate the effect of rheumatoid factor on the determination of serum glycocholic acid and to explore the solution for verifying the test results.Methods The serum samples of different levels of glycocholic acid were diluted with the serum containing high levels of rheumatoid factors.The levels of rheumatoid factors and glycocholic acid in the original and different dilutions of the serum samples were measured.The change curve of the measurement results after dilution was observed to confirm the existence of interference.At the same time,the antinuclear antibody was detected in parallel to observe the interference.Results After adding rheumatoid factor,the serum glycocholic acid decreased,which was positively correlated with the level of rheumatoid factor,and the difference was statistically significant(P<0.05).When the level of rheumatoid factor was below 545 IU/mL,it had no effect on the determination of serum glycocholic acid.With the increase of the level,the interference intensity increased,and the detection result of glycocholic acid decreased sharply;no interference of antinuclear antibody was observed.Conclusion The higher level of rheumatoid factors in serum samples will interfere with the determination of glycocholic acid,which will lead to a false decrease of serum glycocholic acid level,which needs to be paid attention to in clinical practice.In the examination of the results,the results of serum glycocholic acid should be reported by the comprehensive analysis of serum alanine aminotransferase,aspartate aminotransferase and total bile acid.
作者
任鹏
谷春
李建立
刘贵建
REN Peng;GU Chun;LI Jianli;LIU Guijian(Department of Clinical Laboratory,Guang′anmen Hospital,Chinese Academy of Traditional Chinese Medicine,Beijing 100055,China)
出处
《国际检验医学杂志》
CAS
2020年第9期1089-1092,共4页
International Journal of Laboratory Medicine
关键词
类风湿因子
甘胆酸
干扰
抗原抗体反应
rheumatoid factor
glycocholic acid
interference
antigen antibody reaction
