摘要
目的:探究金匮肾气丸对环磷酰胺致小鼠睾丸损伤保护作用及其抗氧化机制. 方法:将30只雄性小鼠均分为空白组、模型组和金匮肾气丸治疗组.治疗组小鼠给予1.2 g/kg体重剂量的金匮肾气丸进行灌胃,空白组和模型组小鼠给予相同体积的生理盐水灌胃,每天1次;在治疗的第7天,模型组和治疗组小鼠给予50 mg/kg体重剂量环磷酰胺进行腹腔注射,每周1次,连续用药35 d.实验结束后对所有小鼠称重(体重和睾丸重量)并采集血液进行睾酮含量检测,取附睾头用于精子质量检测,取睾丸进行组织超氧化物歧化酶(SOD)、丙二醛(MDA),病理组织学及核因子E2相关因子2(Nrf2)信号通路相关基因PCR检测. 结果:实验结束时,模型组小鼠体重[(34.63±1.92) gvs (48.32±1.64)g]、睾丸质量[(80.0±3.9) mg vs (140.0±6.1) mg]、睾丸脏器系数[(0.22±0.01)% vs(0.31±0.03)%]、精子活率[(48.66 ±8.08)% vs (89.33±4.04)%]、精子浓度[(28.42±5.26)×10^6/m1 vs(77.67±8.73)×10^6/ml]、SOD[(140.82±10.08) U/mg prot vs(358.52 ±40.41) U/mg prot]和血清睾酮[(8.75±0.96) pg/ml vs(21.75 ±1.71) pg/ml]水平较空白组显著降低(P<0.05),畸形精子百分率[(37.33±2.08) vs (15.33±1.53)%]和MDA[(54.89±6.09) nmol/ng prot vs(30.21±2.17)nmol/ng prot]水平显著升高(P<0.05);金匮肾气丸治疗组小鼠体重[(39.80±2.89)gvs (34.63±1.92)g]、睾丸质量[(130.00±11.00) mgvs (80.00±3.90) mg]、睾丸脏器系数[(0.28±0.01)%vs(0.22±0.01)%]、精子活率[(76.00±5.29)%vs(48.66±8.08)%]、精子浓度[(56.08±4.29)×10^6/ml vs (28.42 ±5.26)×10^6/ml]、SOD[(206.59±16.38) U/mg prot vs(140.82±10.08) U/mg prot]和血清睾酮[(15.50±1.29) pg/ml vs (8.75±0.96) pg/ml]水平较模型组显著升高(P<0.05),畸形精子百分率[(25.01±2.99)% vs(37.33±2.08)%]和MDA[(35.84±3.61) nmol/ng prot vs (54.89 ±6.09) nmol/ng prot]水平显著降低(P<0.05).模型组依赖还原型辅酶Ⅰ/Ⅱ醌氧化还原酶1(NOQ-1)、Nrf2、血红素氧合酶1(HO-1) mRNA的表�
Objective:To investigate the protective effect of Jinkui Shenqi Pills(JSP)against cyclophospliamide-induced testis injury(TI)and its anti-oxidation mechanism in mice.Methods:Thirty male mice were equally divided into a blank control,a TI model control and a JSP treatment group.The mice in the JSP treatment group were treated intragastrically with JSP and the blank controls with normal saline at 1.2 g/kg qd for 7 days,and then the animals in both the TI model control and JSP treatment groups were injected intraperitoneally with cyclophosphamide at 50 mg/kg,once a week,for 35 days,to induce testis injury.After modeling,all the mice were weighed and sacrificed,followed by detection of the serum T content,measurement of the testis weight,examination of semen parameters in the caudad epididymis,and determination of the levels of super oxide dismutase(SOD)and malondialdehyde(MDA)in the testis tissue and the expressions of relevant genes by qRT-PCR.Results:The mice of the TI model control group,compared with the blank controls,showed significant decreases in the body weight([34.63±1.92][48.32±1.64]g,P<0.05),testis weight([80.00±3.90]仍[140.00±6.10]mg,0.05),testicular organ coefficient([0.22±0.01]vs[0.31±0.03]%,P<0.05),sperm motility([48.66±8.08]%[89.33±4.04]%,P<0.05),sperm concentration([28.42±5.26]vs[77.67±8.73]x 106/ml,P<0.05),and levels of serum T([8.75±0.96]vs[21.75±1.71]pg/m l,P<0.05)and SOD([140.82±10.08]ias[358.52±40.41]U/mg prot,P<0.05),but remarkable increases in the sperm deformity rate([37.33±2.08]vs[15.33±1.53]%,P<0.05)and MDA level([54.89±6.09]vs[30.21±2.17]nmol/ng prot,P<0.05).The mice of the JSP treatment group,in comparison with the TI model controls,exhibited markedly increased body weight([39.80±2.89]vs[34.63±1.92]g,P<0.05),testis weight([130.00±11.00]ws[80.00±3.90]mg,P<0.05),testicular organ coefficient([0.28±0.01]vs[0.22±0.01]%,P<0.05),sperm motility([76.00±5.29]%ws[48.66±8.08]%,P<0.05),sperm concentration([56.08±4.29]vs[28.42±5.26]×10^6/m l,P<0.05),and levels of serum T
作者
蒋平
徐青洪
陈存武
夏伦斌
黄仁术
佘德勇
刘港
晏航
钟枝梅
鲍丽莉
左瑞华
JIANG Ping;XU Qing-hong;CHEN Cun-wu;XIA Lun-bin;HUANG Ren-shu;SHE Dei-yong;LIU Gang;YAN Hang;ZHONG Zhi-mei;BAO Li-li;ZUO Rui-hua(School of Biological and Pharmaceutical Engineering,West Anhui College,Lu Aan,Anhui 237012,China;Engineering Laboratory of Anhui Province for Conservation and Sustainable Utilization of Chinese Medicine Resources,Luan,Anhui 237012,China)
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2020年第2期160-166,共7页
National Journal of Andrology
基金
国家中药材产业技术体系(CARS-21)
国家大学生创新创业训练项目(201810376058)
安徽省高校自然科学项目(KJ2018A0413)
安徽省地方科技创新项目-科技惠民示范工程(202007d07050003)
安徽省大别山等革命老区和贫困县专项(201904f062008)
安徽省本科质量工程项目(2016sjjd079,2018 Zygc075,20l8jyxm1153)
皖西学院自然科学重点项目(WXZR201932)。
关键词
金匮肾气丸
环磷酰胺
睾丸损伤
核因子E2相关因子2
小鼠
Jinkui Shenqi Pills
cyclophosphamide
testis injury
nuclear factor-E2-related factor 2
mouse
