摘要
目的探讨信号通路重要受体基因Notch2及靶蛋白在剥脱综合征白内障患者、健康正常人外周血单个核细胞中的表达水平和意义。方法采用实时荧光定量PCR法检测Notch2基因在18例剥脱综合征白内障患者外周血单个核细胞中的表达水平,同时检测对照组正常人群18例。运用免疫印迹法检测Notch2蛋白在剥脱综合征白内障患者和正常人群中的表达量。结果剥脱综合征患者外周血单个核细胞中Notch2基因相对表达水平为0.157±0.137,正常人群的表达水平为0.035±0.033,剥脱综合征患者显著高于正常健康人群,差异有统计学意义(t=3.662,P<0.05)。免疫印迹检测Notch2蛋白结果显示,剥脱综合征白内障患者蛋白表达量为0.785±0.092,正常人群为0.304±0.091,剥脱综合征患者显著高于正常人群,差异有统计学意义(t=15.795,P<0.01)。结论Notch2基因在剥脱综合征白内障患者外周血单个核细胞中的表达显著高于正常人群,其靶蛋白Notch2在剥脱综合征患者中的表达也高于正常人群。推测作为Notch信号通路重要受体基因Notch2异常表达正调控其靶蛋白Notch2参与剥脱综合征白内障的发病过程。
Objective To investigate the expression and significance of Notch2 gene and its target protein,which in peripheral blood mononuclear cell of patients with exfoliation syndrome cataract and healthy people.Methods There are 18 patients with exfoliation syndrome cataract and 18 healthy people.The expressions of Notch2 was studied with real-time fluorescent quantitative PCR in the peripheral blood mononuclear cell.Western blot was used to detect the expression of Notch2 protein in the peripheral blood mononuclear cell of 18 patients with exfoliation syndrome cataract and 18 normal subjects.Results The expression of Notch2 in peripheral blood mononuclear cell of patients with exfoliation syndrome cataract was 0.157±0.137,significantly higher than the normal population 0.035±0.033,and the difference was statistically significant(t=3.662,P<0.05).Western blot showed that the expression of Notch2 protein in patients with exfoliation syndrome cataract were 0.785±0.092,and in normal people were 0.304±0.091.The expression of Notch2 protein in exfoliation syndrome cataract patients was higher than in normal people.And the differences was statistical significance(t=15.795,P<0.01).Conclusion The expression of Notch2 and its target protein in peripheral blood mononuclear cell of patients with exfoliation syndrome cataract was significantly higher than the normal people.Differential expression of Notch2 may positive regulation of Notch2 protein in the development of exfoliation syndrome eye disease.
作者
丁琳
王慧琴
凯迪丽亚·阿力甫
买尔哈巴·米吉提
吴卫东
Ding Lin;Wang Huiqin;Kai Diliyaalifu(People′s Hospital of Xingjiang Uygur Autonomous Region,Xinjiang 830001,China)
出处
《医学研究杂志》
2020年第4期105-108,共4页
Journal of Medical Research
基金
新疆维吾尔自治区自然科学基金资助项目(2017D01C117)。
