摘要
为探究KNOX(KNOTTED1-like homeobox)家族基因在烟草叶片发育中的功能,从普通烟草(Nicotiana tobacum)红花大金元中克隆获得KNOX家族基因NtabKNOX8,对其进行了生物信息学分析,并通过转基因技术获得了NtabKNOX8过表达材料,对转生长素报告基因DR5::GUS材料与NtabKNOX8过表达材料的杂交F1代叶片进行了GUS染色。结果表明,NtabKNOX8属于KNOX基因家族亚家族Ⅰ;过表达NtabKNOX8导致叶片变宽圆,对称性丧失,叶面皱缩并向叶背面弯曲,主脉明显缩短且脉序发生明显改变,类似掌状网脉,叶形指数变小,叶面积减小,比叶鲜重增大;NtabKNOX8过量表达的烟叶中生长素含量明显增加,且分布更加广泛。推测NtabKNOX8可能通过影响生长素的含量及分布,影响叶片生长发育过程,从而导致了烟叶形态的多重变化。
In order to explore the function of KNOX family genes in tobacco leaf development,a KNOX family gene NtabKNOX8 was cloned from Nicotiana tobacum cv.Honghuadajinyuan and bioinformatics analysis was performed.Transgenic technique was performed to obtain NtabKNOX8 over-expression(OE)lines.And then GUS staining experiments were performed on the leaves of the hybrids(F1)of materials transferred into the auxin reporter DR5::GUS and materials overexpressing NtabKNOX8.The results showed that NtabKNOX8 belongs to the KNOX family subfamilyⅠ.Compared to the wild type tobacco plants,the NtabKNOX8 OE lines showed round and wrinkled leaves which curved toward the back and lost leaf symmetry.Meanwhile,the midrib of the two OE lines was greatly shortened and the venation changed significantly to palmately-netted venation-like type,with the leaf shape index and the leaf area decreased,leaf specific fresh weight increased.In addition,the auxin content was significantly increased while its distribution was more widely and extensive in the tobacco leaves overexpressing NtabKNOX8.It is suggested that NtabKNOX8 may regulate the growth and development of tobacco leaves by affecting the content and distribution of auxin,which finally leads to multiple changes in tobacco leaves.
作者
徐方正
史素娟
毛静静
安璐璐
刘贯山
刘好宝
王倩
XU Fangzheng;SHI Sujuan;MAO Jingjing;AN Lulu;LIU Guanshan;LIU Haobao;WANG Qian(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《中国烟草科学》
CSCD
北大核心
2020年第2期86-92,共7页
Chinese Tobacco Science
基金
中国农业科学院烟草研究所青年科学基金“青岛地区果蔬作物上马铃薯卷叶病毒属病毒的分子鉴定”(2016B02)
山东省自然科学基金“烟草MATE家族基因NtDSI在生长素转运过程中的功能解析及上游调控机制研究”(ZR2017QC003)
中国烟草总公司重大专项“特征香韵烤烟突变模块解析与工业可用性研究”[110201801028(JY-05)]。
