摘要
KNOX基因家族是具有同源异型盒结构域的转录因子,在植物形态建成、模式形成等过程中起重要调控作用。为明确烟草KNOX基因的结构及生物学功能,分别从普通烟草、林烟草和绒毛状烟草中鉴定出18、8和5个KNOX基因,并对其系统进化、基因结构、蛋白功能域及基因表达模式进行分析。结果表明,烟草KNOX基因可分为NtKNOXⅠ和NtKNOXⅡ两个亚类,两亚类又进一步分为5个进化分支。同一进化分支中烟草KNOX蛋白的功能域以及其编码序列在基因结构上的分布高度保守。基因表达分析表明,普通烟草中NtKNOXⅡ类基因在多个组织中表达,而且整体表达水平高于NtKNOXⅠ类基因。在林烟草和绒毛状烟草的根和叶中表达量最高的KNOX基因不同。
KNOX genes encode transcription factors with homeobox domains,which play important roles in plant morphogenesis and pattern formation.To explore the structure and functions of KNOX genes from tobacco,18,8 and 5 KNOX genes were identified from Nicotiana tabacum,N.sylvestris and N.tomentosiformis,respectively.In addition,their phylogenetic,gene structure,protein functional domains and gene expression patterns were analyzed.The results showed that KNOX genes from tobacco could be divided into two subclasses:NtKNOXⅠand NtKNOXⅡ,and the two subclasses were further divided into 5 evolutionary branches.In the same evolutionary branch,the functional domains of tobacco KNOX proteins and their coding sequences were highly conserved in gene structure.Expression analysis indicated that NtKNOXⅡgenes in N.tabacum were expressed in multiple tissues,and the overall expression level was higher than that of NtKNOXⅠgenes.The high expressed genes were different in roots and leaves of N.sylvestris and N.tomentosiformis.
作者
刘向真
王根发
金立锋
张林
魏攀
王晨
李泽锋
王宇博
王燃
杨永锋
王中
LIU Xiangzhen;WANG Genfa;JIN Lifeng;ZHANG Lin;WEI Pan;WANG Chen;LI Zefeng;WANG Yubo;WANG Ran;YANG Yongfeng;WANG Zhong(Technology Center,China Tobacco Henan Industrial Co.,Ltd.,Zhengzhou 450000,China;Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;College of Tobacco Science,Henan Agricultural University,Zhengzhou 450002,China;College of Life Science,Zhengzhou University,Zhengzhou 450001,China)
出处
《烟草科技》
EI
CAS
CSCD
北大核心
2019年第2期1-11,共11页
Tobacco Science & Technology
基金
中国烟草总公司郑州烟草研究院院长基金项目"烟草转录因子NtMYB27调控类黄酮合成流向的功能研究"(902017CA0220)
