摘要
植物咖啡酸-O-甲基转移酶(caffeic acid O-methyltransferase,COMT)基因对木质素的合成具有重要的调控作用。本研究采用生物信息学方法对芍药等8种植物COMT基因编码的氨基酸序列进行比对,进而对其蛋白理化性质、信号肽、跨膜结构域、磷酸化位点、亚细胞定位、二级结构与三级结构进行了预测与分析。结果表明,芍药等8种植物COMT基因编码氨基酸的一致性可达65.91%,平均相对分子量为39437.79 Da,带有酸性等电点,含量最丰富的氨基酸为亮氨酸(Leu)和丙氨酸(Ala),无信号肽,无跨膜结构域,磷酸化位点数量最多的为丝氨酸和苏氨酸,定位于细胞骨架和细胞质中可能性最大,二级结构则以α-螺旋和无规则卷曲为主,三维结构具有一定的相似性。这一结果为今后深入开展植物COMT基因功能研究提供了科学依据。
The plant caffeic acid O-methyltransferase(COMT)gene plays an important role in regulating lignin synthesis.In this study,bioinformatics methods were used to compare the amino acid sequences encoded by the COMT genes of 8 plants including herbaceous peony,and then to analyze their protein physicochemical properties,signal peptides,transmembrane domains,phosphorylation sites,subcellular localization,and secondary structure and tertiary structure.The results showed that the identity of the amino acids encoded by COMT gene of 8 plants including herbaceous peony reached 65.91%,the average relative molecular weight was 39437.79 Da with acid isoelectric point,and the most abundant amino acids were leucine(Leu)and alanine(Ala),no signal peptide and no transmembrane domain were found.The most phosphorylation sites were serine and threonine,most likely localized in the cytoskeleton and cytoplasm,and the secondary structure was dominated byα-helix and random coil,three-dimensional structure simulation had a certain similarity.This result laid the foundation for further research on the function of plant COMT gene in the future.
作者
许聪
石文波
高天祥
胡蕴钰
陶俊
赵大球
Xu Cong;ShiWenbo;Gao Tianxiang;Hu Yunyu;Tao Jun;Zhao Daqiu(College of Horticulture and Plant Protection,Yangzhou University,Yangzhou,225009)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第8期2468-2474,共7页
Molecular Plant Breeding
基金
国家自然科学基金(31972448
31772341)
江苏省科协青年科技人才托举工程
扬州大学研究生科研创新计划(XKYCX19_114)
扬州大学大学生科创基金(X20190595)共同资助。
