摘要
为研究苯丙氨酸解氨酶(PAL)作为苯丙烷类代谢途径的关键酶,在植物响应环境胁迫过程中的重要作用,本研究利用HMMER、Pfam与SMART等工具,从拟南芥全基因组中筛选获得9个PAL成员,分析其编码酶蛋白的理化性质、二级结构与保守基序等信息.同时,结合转录组与定量PCR分析PAL成员在干旱与盐胁迫条件下的表达模式.结果表明,拟南芥PAL成员的二级结构主要由α-螺旋和无规则卷曲组成;亮氨酸和丙氨酸为含量较高的氨基酸残基;拟南芥PAL成员含有20种不同的保守基序,其中motif6含有ASG活性位点,为所有PAL成员所共有;较多PAL成员具有光响应、激素响应、胁迫响应和生长发育相关的顺式调控元件.转录组学与定量PCR分析发现,AT3G53260.1、AT3G53260.2与AT3G47660.1的mRNA表达水平在干旱与盐胁迫后发生显著变化,推测这3条基因可能参与拟南芥对干旱及盐胁迫的响应过程,表明PAL基因在调控植物生长发育与非生物胁迫响应过程中发挥重要作用.
To study the important role of phenylalanine ammonia-lyase,as a key enzyme in the phenylpropanoid metabolic pathway,in plant response to environmental stresses,the genomic information of Arabidopsis thaliana was downloaded from Phytozome and the PAL members were further identified by HMMER,Pfam and SMART tools.The physicochemical properties,secondary structure and conserved motifs of nine PAL proteins were analyzed.The results showed that the secondary structure of PAL members in Arabidopsis thaliana was mainly composed ofα-helix and random curl.Leu and Ala were the most abundant amino acid residues.There were 20different conserved motifs in the PALgene family of Arabidopsis thaliana,among which motif 6containing active site ASG appeared in all PALgenes.The analysis of cis-acting elements showed that the PALgene family contained many elements related to light response,hormone response,stress response and growth and development.The mRNA expression levels of AT3G53260.1,AT3G53260.2and AT3G47660.1showed obvious changes based on transcriptome analysis,and the corresponding tendency was verified by further real-time PCR.Therefore,these3genes might be involved in the response to drought and salt stress of Arabidopsis thaliana.These results indicate that PAL genes play an important role in regulating plant growth and development and abiotic stress response.
作者
秦余
田春尧
赵咏洋
黄思沛
宋思敏
廖海
QIN Yu;TIAN Chun-Yao;ZHAO Yong-Yang;HUANG Si-Pei;SONG Si-Min;LIAO Hai(School of Life and Science,Southwest Jiaotong University,Chengdu 610031,China)
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2022年第6期166-172,共7页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金青年科学基金项目(31500276)
四川省中医药管理局项目(2020JC0128
2021MS116)
中央高校基本科研业务费医工结合项目(2682021ZTPY017)
国家级大学生创新创业训练计划资助项目(202110613083)
西南交通大学个性化实验项目(GX20211600310001)。
关键词
拟南芥
苯丙氨酸解氨酶
基因家族
生物信息学分析
Arabidopsis thaliana
Phenylalanine ammonia-lyase
Gene family
Bioinformatics analysis
