摘要
目的:建立黄花柳花中芦丁、木犀草素-7-О-β-D-吡喃葡萄糖苷、芹菜素-7-O-β-D-吡喃葡萄糖苷、木犀草素、芹菜素的HPLC分析方法。方法:采用Agilent TC-C18色谱柱(4.6mm×250mm,5μm),以乙腈-0.2%磷酸溶液梯度洗脱,检测波长340nm,流速0.8mL/min,柱温30℃。结果:芦丁、木犀草素-7-О-β-D-吡喃葡萄糖苷、芹菜素-7-O-β-D-吡喃葡萄糖苷、木犀草素、芹菜素的线性范围分别为8.11~121.68μg/mL(r=0.9994),2.92~43.80μg/mL(r=0.9995),4.13~61.92μg/mL(r=0.9995),2.04~30.60μg/mL(r=0.9995),2.05~30.78μg/mL(r=0.9996);平均回收率(n=9)分别为100.25%(RSD=1.60%),98.24%(RSD=1.23%),101.57%(RSD=1.25%),98.45%(RSD=1.42%),101.30%(RSD=1.17%)。结论:本方法操作简便,快速准确,可以用于黄花柳花中黄酮类成分的定量分析。
Objective:To establish a high performance liquid chromatography (HPLC) method for the determination of Rutin,luteolin-7-O-β-D-pyranoside,apigenin-7-O-β-D-pyranoside,luteolin and apigenin in flower of Salix caprea L..Methods:Agilent TC-C18 column (4.6mm×250mm,5μm) was used to elucidate with acetonitrile-0.2% phosphoric acid solution.The detection wavelength was 340nm,the flow rate was 0.8mL/min,and the column temperature was 30℃.Results:The linear ranges of rutin,luteolin-7-O-β-D-pyranoside,apigen-7-O-β-D-pyrrolidine,luteolin and apigenin were 8.11~121.68μg/mL (r=0.9994),2.92~43.80μg/mL (r=0.9995),4.13~61.92μg/mL(r=0.9995),2.04~30.60μg/mL (r=0.9995),and 2.05~30.78μg/mL(r=0.9996) respectively.The average recovery (n=9) was 100.25% (RSD=1.60%),98.24% (RSD=1.23%),101.57% (RSD=1.25%),98.45% (RSD=1.42%) and 101.30% (RSD=1.17%) respectively.Conclusion:The method established in this article is simple,rapid and accurate,and can be used for quantitative analysis of flavonoids in flower of Salix caprea L..
作者
刘倩芸
迪娜·吐尔洪
李新霞
许芳
李建光
LIU Qian-yun;Dina·Tuerhong;LI Xin-xia;XU Fang;LI Jian-guang(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2020年第1期411-414,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
新疆维吾尔自治区重点研发项目(No.2016B03044-4)
省部共建中亚高发病成因与防治国家重点实验室项目(No.SKL-HIDCA-2018-11)。
