摘要
采用超声波辅助法提取炮仗花类胡萝卜素,采用分光光度法测定其含量,通过红外光谱对其结构进行表征,并利用脂多糖(LPS)诱导的小鼠巨噬细胞(RAW264.7)炎症模型评价其抗炎活性。结果表明,最佳提取方法为:以20 mL无水乙醇为提取溶剂,超声波辅助提取20 min。β-胡萝卜素浓度(x,μg·mL^-1)在0.996~3.486μg·mL^-1范围内与吸光度(y)线性关系良好,其线性回归方程为y=0.1461x-0.0207,R2=0.9999(n=6),精密度RSD为0.19%,稳定性RSD为0.59%,重复性RSD为0.74%,平均加样回收率为100.08%,RSD为1.46%(n=9),测得炮仗花类胡萝卜素含量为31.77μg·mg^-1。抗炎活性实验表明,炮仗花类胡萝卜素在高浓度时呈一定细胞毒性,且浓度依赖性抑制NO生成,具有一定的抗炎活性。
We extracted carotenoid from Pyrostegia venusta by an ultrasonic-assisted method,determined its content by spectrophotometry,characterized its structure by FTIR,and evaluated its anti-inflammatory activity using a LPS-induced mouse macrophage(RAW264.7)inflammatory cell model.The results show that the optimal extraction method is determined as follows:using 20 mL absolute ethanol as the extraction solvent,ultrasonic-assisted extraction for 20 min.There is a good linear relationship ofβ-carotene concentration(x,μg·mL^-1)with absorbance(y)in the range of 0.996-3.486μg·mL^-1,and the linear regression equation is y=0.1461x-0.0207,R2=0.9999(n=6).RSDs of the precision,stability,and repeatability are 0.19%,0.59%,and 0.74%,respectively.The average adding standard recovery is 100.08%,and the RSD is 1.46%(n=9).The carotenoid content in Pyrostegia venusta is 31.77μg·mg^-1.Furthermore,the anti-inflammatory activity experiment shows that the carotenoid from Pyrostegia venusta inhibites NO production in a dose-dependent manner and has certain cytotoxicity at high dose,which indicates the carotenoid from Pyrostegia venusta has certain anti-inflammatory activity.
作者
林启凰
林聪炜
张娜
纪美茹
陈仲巍
张岗
LIN Qihuang;LIN Congwei;ZHANG Na;JI Meiru;CHEN Zhongwei;ZHANG Gang(Department of Pharmacy,Xiamen Medical College,Xiamen 361000,China;Xiamen Humanity Hospital,Xiamen 361000,China)
出处
《化学与生物工程》
CAS
2020年第3期42-47,共6页
Chemistry & Bioengineering
基金
福建省教育厅中青年教师教育科研项目(JAT170705)
厦门医学院自然科学类项目(K2016-19)。
关键词
炮仗花
类胡萝卜素
含量测定
分光光度法
抗炎活性
Pyrostegia venusta
carotenoid
content determination
spectrophotometry
anti-inflammatory activity
