摘要
目的观察膜联蛋白A1拟肽Ac2-26对体外循环大鼠肺损伤及中性粒细胞凋亡的影响。方法24只雄性SD大鼠按随机数字表法分为3组(n=8):假手术组(S组)、缺血再灌注组(IR组)、Ac2-26组(A组)。假手术组行股动、静脉,颈总动脉穿刺置管,不行体外循环;另外两组建立体外循环左肺缺血再灌注损伤模型,在体外循环开始后并循环10 min时开胸,阻断左肺门,45 min后恢复灌注。缺血再灌注组、Ac2-26组分别于阻断左肺门前30 min静脉给予等容量0.9%NaCl、AnxA1拟肽Ac2-26(1 mg/kg),分别在CPB开始前即刻(T 1)、开放左肺门即刻(T 2)、CPB结束后1.5 h时(T 3)采集动脉血,测动脉血气并计算氧合指数(OI)、呼吸指数(RI)、肺泡动脉氧分压差(P(A-a)O 2),T 3时点取左肺支气管肺泡灌洗液(BALF)及左肺组织,ELISA检测肺组织中TNF-α、IL-10和BALF中IL-6、总蛋白的含量,TUNEL检测肺组织中性粒细胞的凋亡水平,Western blot检测肺组织AnxA1的表达。结果T 3时点,与假手术组比较,缺血再灌注组OI降低、RI和P(A-a)O 2升高,肺组织病理结构损伤明显,肺损伤评分明显升高(P<0.05);与缺血再灌注组比较,Ac2-26组OI升高,RI、P(A-a)O 2明显下降,肺组织中TNF-α和BALF中IL-6、总蛋白含量明显降低,肺组织中IL-10含量、AnxA1的表达及肺组织中性粒细胞的凋亡率明显高于缺血再灌注组,肺组织病理结构明显改善,病理损伤评分显著下降(P<0.05)。结论膜联蛋白A1拟肽Ac2-26可增加大鼠肺组织内源性AnxA1的表达及IL-10含量,促进肺中性粒细胞凋亡,减轻体外循环肺损伤,对肺有一定的保护作用。
Objective To observe the effects of Annexin A1 peptide Ac2-26 on lung injury and neutrophil apoptosis in rats with cardiopulmonary bypass.Methods Twenty-four male SD rats were randomly divided into three groups(n=8):Sham operation group(S group),Ischemia-Reperfusion group(IR group),and Ac2-26 group(A group).In the S group,femoral artery,vein and common carotid artery were punctured and catheterized without cardiopulmonary bypass;in the other two groups,left lung ischemia-reperfusion injury model was established.After cardiopulmonary bypass for 10 minutes,chest was opened and left lung hilus was blocked.And then perfusion was resumed 45 minutes later.In the IR and A groups,0.9%NaCl and AnxA1 peptide Ac2-26(1 mg/kg)were given intravenously 30 minutes before the occlusion of left pulmonary hilum respectively.Arterial blood was collected immediately before CPB(T 1),immediately after the opening of left pulmonary hilum(T 2),and 1.5 hours after CPB(T 3).Arterial blood gas analysis was carried out,and Oxygenation Index(OI),Respiratory Index(RI)and Alveolar arterial oxygen differential pressure were calculated.At the time of T 3,BALF and left lung tissues were collected.TNF-α,IL-10,IL-6 and total protein in BALF were detected by ELISA,apoptosis of neutrophils in lung tissues was detected by TUNEL,and the protein level of AnxA1 was detected by Western blot.Results At the T 3 time point,OI was decreased,and RI and P(A-a)O 2 were increased in the IR group compared with the S group.The pathological structure damage of lung tissue was obvious,and lung injury score was significantly increased(P<0.05).Compared with the IR group,OI was increased in the A group.RI and P(A-a)O 2 were decreased.The apoptosis rate of neutrophils was significantly higher than that of the IR group.The pathological structure of lung tissue was significantly improved,and the pathological damage score was significantly decreased(P<0.05).Conclusion Ac2-26 can increase the expression of AnxA1 and the content of IL-10,promote the apoptosis of pulmonary neu
作者
张元杰
吴汉华
罗俊丽
郭宇含
程翅
张红
Zhang Yuanjie;Wu Hanhua;Luo Junli;Guo Yuhan;Cheng Chi;Zhang Hong(School of Graduate,Zunyi Medical University,Zunyi Guizhou 563099,China;School of Anesthesiology,Zunyi Medical University,Zunyi Guizhou 563099,China)
出处
《遵义医科大学学报》
2020年第1期21-26,共6页
Journal of Zunyi Medical University
基金
国家自然科学基金资助项目(NO:81760079)。
