摘要
目的评价乌司他丁预先给药对体外循环(CPB)大鼠急性肺损伤时水通道蛋白1(AQP1)和AQP5表达的影响。方法清洁级健康成年雄性SD大鼠48只,体重200~250g,采用随机数字表法分为3组(n=16):假手术组(Sham组)、体外循环组(CPB组)和UTI组(UTI组),每组16只。UTI组大鼠于CPB前10min静脉注射乌司他丁溶液20万U/kg;CPB组和UTI组建立CPB模型,Sham组和CPB组大鼠分别于动脉和静脉穿刺前10min或CPB前10min静脉注射等容量生理盐水。CPB结束1h时处死大鼠取肺,称重,计算湿重/干重(W/D)比值,光镜下观察肺组织形态结构并计算肺泡损伤率(IAR),电镜下观察肺组织超微结构。采用免疫组化法检测AQP1和AQP5的表达水平。采用Westernblot法和RT-PCR法分别测定AQP1和AQP5及其mRNA的表达水平。结果与Sham组比较,CPB组和UTI组肺组织W/D比值和IAR升高,AQP1和AQP5阳性细胞率降低,AQP1和AQP5及其mRNA表达下调(P<0.05);与CPB组比较,UTI组肺组织W/D比值和IAR降低,AQP1和AQP5阳性细胞率升高,AQP1和AQP5及其mRNA表达上调(P<0.05)。UTI组形态学和超微结构损伤较CPB组减轻。结论乌司他丁预先给药减轻CPB大鼠急性肺损伤的机制与其上调AQP1和AQP5的表达有关。
Objective To evaluate the effect of ulinastatin(UTI)on the expression of aquaporin 1(AQP1)and AQP5 in rats with acute lung injury induced by cardiopulmonary bypass(CPB). Methods Forty-eight clean-grade healthy adult male Sprague-Dawley rats, weighing 200-250 g, were divided into 3 groups(n=16 each)using a random number table method: sham operation group(Sham group), CPB group and UTI group.UTI 200 000 U/kg was injected intravenously at 10 min prior to CPB in UTI group.The model of CPB was established in CPB and UTI groups.The equal volume of normal saline was intravenously injected at 10 min prior to puncture or at 10 min prior to CPB in Sham and CPB groups.Rats were sacrificed, and lung tissues were excised for determination of weight to dry weight ratio(W/D ratio), expression of AQP1 and AQP5(by immunohistochemistry), expression of AQP1 and AQP5 protein and mRNA(by real-time polymerase chain reaction or Western blot)and for examination of morphological structure(with a light microscope)and ultrastructure of lung tissues(with an electron microscope). Injured alveolar rate(IAR)and rates of AQP1 and AQP5 positive cells were calculated. Results Compared with Sham group, W/D ratio and IAR were significantly increased, rates of AQP1 and AQP5 positive cells were decreased, and the expression of AQP1 and AQP5 protein and mRNA was down-regulated in CPB and UTI groups (P<0.05). Compared with CPB group, W/D ratio and IAR were significantly decreased, rates of AQP1 and AQP5 positive cells were increased, and the expression of AQP1 and AQP5 protein and mRNA was up-regulated in UTI group(P<0.05). The injury to morphological structure and ultrastructure was significantly attenuated in UTI group when compared with CPB group. Conclusion The mechanism by which UTI pretreatment reduces CPB-induced acute lung injury is related to up-regulating the expression of AQP1 and AQP5 in rats.
作者
郎志斌
范晓珍
林洪启
邱林
张加强
高传玉
Lang Zhibin;Fan Xiaozhen;Lin Hongqi;Qiu Lin;Zhang Jiaqiang;Gao Chuanyu(Department of Anesthesiology , People′s Hospital of Zhengzhou University, Henan Provincial People′s Hospital, Fuwai Central China Cardiovascular Hospital, Zhengzhou 450003, China;Laboratory Medicine, People′s Hospital of Zhengzhou University, Henan Provincial People′s Hospital, Fuwai Central China Cardiovascular Hospital, Zhengzhou 450003, China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2018年第10期1261-1265,共5页
Chinese Journal of Anesthesiology
