摘要
目的通过检测子痫前期孕妇胎盘组织中Th22、CD4+CD25+CD127low Treg细胞转录因子AHR、Foxp3及细胞因子IL-22、IL-10的表达,探讨其在子痫前期中的作用。方法选取35例明确诊断的子痫前期孕妇为研究组,同时选取正常妊娠孕妇35例为对照组,采用实时荧光定量PCR检测两组孕妇胎盘组织中Th22、CD4+CD25+CD127low Treg细胞转录因子AHR mRNA、Foxp3 mRNA的表达情况;采用免疫组化检测细胞因子IL-22、IL-10的表达。结果AHR mRNA在研究组和对照组的表达量分别为(1.85±0.47)和(0.89±0.26),Foxp3 mRNA在两组的表达量分别为(0.76±0.23)和(1.31±0.42),差异均具有统计学意义;IL-22在研究组和对照组的阳性表达率分别为68.57%和40.00%,IL-10在两组的表达率为34.29%和60.00%,差异也具有统计学意义。结论Th22、Treg细胞转录因子及细胞因子的异常表达,可能参与了子痫前期的发生。
Objective To investigate the expression of Th22,CD4+CD25+CD127low Treg cell transcription factors AHR,Foxp3,and cytokines IL-22 and IL-10 in the placenta of preeclampsia pregnant women,and to explore their role in preeclampsia.Methods Thirty-five definite pregnant women with preeclampsia were selected as the study group,while 35 normal pregnant women were selected as the control group.The Th22,CD4+CD25+CD127low Treg cell transcription factor AHR mRNA and Foxp3 mRNA expressions in placental tissues of two groups of pregnant women were detected by real-time fluorescence quantitative PCR.The cytokine IL-22 and IL-10 expressions were detected by immunohistochemical method.Results The expression levels of AHR mRNA in the study group and control group were(1.85±0.47)and(0.89±0.26),respectively.The expression levels of Foxp3 mRNA in the two groups were(0.76±0.23)and(1.31±0.42),and the differences were statistically significant.The positive expression rates of IL-22 in the study group and control group were 68.57%and 40.00%,respectively.The expression rates of IL-10 in the two groups were 34.29%and 60.00%,and the differences were also statistically significant.Conclusion The abnormal expression of Th22 and Treg cell transcription factors and cytokines may be involved in the occurrence of preeclampsia.
作者
王连云
张琼
金栩冰
熊文栋
叶文
王繁
WANG Lianyun;ZHANG Qiong;JIN Xubing;XIONG Wendong;YE Wen;WANG Fan(Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou325027,China)
出处
《中国现代医生》
2020年第5期38-41,共4页
China Modern Doctor
基金
浙江省温州市科技局基础性科研项目(Y20180304)
浙江省自然科学基金资助项目(LY15H040012)
浙江省自然科学基金资助项目(LY18H040011)。
