摘要
目的研究子宫内膜异位症(E MT)患者血清及腹腔液中白细胞介素-37(IL-37)水平变化与肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)的相关性,探讨IL-37在EMT发病机制中的作用。方法选取中南大学湘雅三医院妇科2018年7月-2019年1月收治的经腹腔镜手术及术后病理证实为EMT的患者36例为EMT组(早期13例,晚期23例),选取同期因单纯性卵巢囊肿、畸胎瘤或子宫肌瘤在妇科行腹腔镜手术治疗的患者28例为对照组。采用酶联免疫吸附法测定两组患者血清和腹腔液中的IL-37、TNF-α、IFN-γ水平并进行组间比较,同时分析IL-37与TNF-α、IFN-γ的相关性。结果EMT组患者血清IL-37、TNF-α水平明显高于对照组[分别为(101.78±31.58)pg/mlvs.(63.50±14.48)pg/ml、(30.68±16.22)pg/mlvs.(18.09±1.27)pg/ml],腹腔液中的IL-37、TNF-α水平明显高于对照组[分别为(133.94±47.07)pg/mlvs.(68.43±12.60)pg/ml、(24.23±6.12)pg/mlvs.(16.63±3.13)pg/ml];EMT组患者血清及腹腔液中的IFN-γ水平明显低于对照组[分别为(18.81±3.20)pg/ml vs.(20.52±1.38)pg/ml、(15.93±2.58)pg/ml vs.(20.32±7.03)pg/ml],差异有统计学意义(P<0.05)。随病情加重,EMT组患者血清及腹腔液中IL-37、TNF-α的表达量呈上升趋势,IFN-γ呈下降趋势。在EMT组中,血清及腹腔液中IL-37水平与TNF-α呈正相关(r分别为0.338、0.518,P<0.05),与IFN-γ呈负相关(r分别为–0.332、–0.370,P<0.05)。在对照组中,血清及腹腔液中IL-37水平与TNF-α、IFN-γ均不存在相关关系。结论EMT患者血清及腹腔液中IL-37水平明显增高,可能通过促进TNF-α及抑制IFN-γ的分泌在EMT发生发展中起一定作用。
Objective To investigate the relationship of tumor necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)to interleukin-37(IL-37)in the serum and peritoneal fluid,and investigate the pathogenesis of IL-37 in endometriosis(EMT)patients.Methods Thirty-six EMT patients,admitted in the Department of Obstetrics and Gynecology,the Third Xiangya Hospital of Central South University from Jul,2018 to Jan.2019,and confirmed by laparoscopic operation and pathology,were selected as the EMT group,of which 13 cases were in stageⅠandⅡ,and 23 cases in stageⅢandⅣ.The other 28 women with simple ovarian cyst,teratoma or hysteromyoma were selected as the control group.ELISA was performed to detect the levels of IL-37,TNF-αand IFN-γin serum and abdominal fluid of the two groups.And then the levels of IL-37,TNF-αand IFN-γwere compared between the two groups and their relationship was analyzed.Results The serum levels of IL-37 and TNF-αwere significantly higher in EMT group than those in control group[(101.78±31.58)pg/ml vs.(63.50±14.48)pg/ml and(30.68±16.22)pg/ml vs.(18.09±1.27)pg/ml,respectively].The levels of IL-37 and TNF-αin peritoneal fluid were significantly higher in EMT group than those in control group[(133.94±47.07)pg/ml vs.(68.43±12.60)pg/ml and(24.23±6.12)pg/ml vs.(16.63±3.13)pg/ml,respectively].The levels of IFN-γin serum and peritoneal fluid were significantly lower in EMT group than that in control group[(18.81±3.20)pg/ml vs.(20.52±1.38)pg/ml and(15.93±2.58)pg/ml vs.(20.32±7.03)pg/ml,respectively].The differences were statistically significant(P<0.05).In EMT group,the expressive levels of IL-37 and TNF-αincreased,and of IFN-γdecreased in serum and peritoneal fluid with the aggravation of the disease.In EMT group,the IL-37 levels in serum and peritoneal fluid were positively correlated with the TNF-αlevels(r=0.338 and 0.518 respectively,P<0.05),while negatively correlated with the IFN-γlevels(r=–0.332 and–0.370 respectively,P<0.05).In control group,no significant correlation existed in IL-
作者
江昭颖
蒋建发
薛敏
Jiang Zhao-Ying;Jiang Jian-Fa;Xue Min(Department of Obstetrics and Gynecology,the Third Xiangya Hospital of Central South University,Changsha 410013,China)
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2020年第3期304-307,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金青年科学基金(81801422)。
