摘要
目的探讨子宫内膜异位症（endometriosis,EMs）不孕患者腹腔液中的微生物菌群及其肿瘤坏死因子α（TNF-α）和白介素37（IL-37）水平的变化。方法采集内膜异位症不孕组（T）29例和对照组（C）19例女性的腹腔液（peritoneal fluid,PF）标本,分别进行基因组DNA提取后Ion Torrent PGM平台测序检测微生物菌群和酶联免疫吸附试验（ELISA）检测IL-37及TNF-α水平。结果 EMs不孕患者和对照组女性PF中均发现了多种菌群存在,在菌门水平上主要分为变形菌门Proteobacteria、厚壁菌门Firmicutes、拟杆菌门Bacteriodetes、放线菌门Actinobacteria、梭杆菌门Fusobacteria,其中Proteobacteria为主要菌群,占60%以上的序列,软壁菌门Tenericutes只在对照组中检出1例,各菌门在两组间差异无统计学意义（P〉0.05）。EMs不孕妇女腹腔液中TNF-a和IL-37水平分别为（75.87±8.47）pg/ml、（372.03±68.02）pg/ml,高于对照组（57.76±8.29）pg/ml和（261.37±52.78）pg/ml（P〈0.05）;EMs不孕组Ⅲ-Ⅳ期TNF-a的水平为（83.47±4.73）pg/ml,显著高于Ⅰ-Ⅱ期（74.29±8.26）pg/ml（P〈0.05）,IL-37的水平在二者中差异无统计学意义（P〉0.05）;内膜异位症不孕组中两者存在正相关关系（r=0.569,P〈0.001）,对照组中两者不存在相关关系（r=-0.247,P=0.522）。结论 EMs不孕患者和对照组女性腹腔液中存在多种菌群,EMs不孕组中TNF-a和IL-37含量增高可能在EMs不孕症的发生机制中起到重要作用。
Objective To study on microbiome and concentration changes of tumor necrosis factoralpha（TNF-α）and interleukin-37（IL-37）in peritoneal fluid（PF）of infertility women with endometriosis. Methods Peritoneal fluid from 29 infertile women with endometriosis and a control group of 19 fertile women were detected for TNF-α and IL-37 levels by ELISA and for DNA extracting and Ion Torrent PGM sequencing respectively. Results Peritoneal fluid microbiome were divided into five phyla including Proteobacteria,Firmicutes,Bacteriodetes,Actinobacteria,Fusobacteria. Proteobacteria was the dominant flora which contained over 60% sequences and Tenericutes was detected only in one case in the control group. There was no significantly differences of abundances of six phyla between the two groups（P 0.05）.TNF-α and IL-37 concentrations were increased in infertile women with endometriosis[（75.87±8.47）;（372.03±68.02）pg/ml]versus controls[（57.76±8.29）;（261.37±52.78）pg/ml];P 0.05）;In infertile women with stage Ⅲ and stage Ⅳ endometriosis,the levels of TNF-α was（83.47±4.73）pg/ml,which was significantly higher than those of stages Ⅰ and Ⅱ（74.29±8.26）pg/ml（P 0.05）.This trend was not seen in IL-37（P 0.05）.The concentrations of TNF-α and IL-37 in PF of endometriosis-associated infertile women had positive correlation with each other（r = 0.569,P 0.001）,whereas this trend was not seen in control group（r=-0.247,P = 0.522）.Conclusion There was a diversity of bacteria in PF of infertile women associated with EMs and control group andhigher levels of TNF-α and IL-37 in PF may play an important role in pathogenesis of infertile women with EMs.
出处
《中国妇产科临床杂志》
CSCD
北大核心
2015年第5期413-416,共4页
Chinese Journal of Clinical Obstetrics and Gynecology
