摘要
目的探讨心脏过表达三酰甘油脂肪酶(ATGL)对烧伤后小鼠心肌损害的影响及可能机制。方法随机选取8周龄心肌肌球蛋白重链(MHC)启动子驱动的心脏过表达ATGL雄性小鼠(MHC-ATGL烫伤组)和野生型雄性C57BL/6J小鼠(野生型烫伤组)构建烫伤模型,以同周龄和同性别的MHC-ATGL假烫伤小鼠(MHC-ATGL对照组)和野生型C57BL/6J假烫伤小鼠(野生型对照组)作为对照,每组8只。烫伤24 h后取材进行实验。分别检测心脏组织中ATGL蛋白表达水平、血清心肌肌钙蛋白T、肌酸激酶同工酶、心脏组织游离脂肪酸含量和心脏组织活性氧水平。两烫伤组分别与其对照组进行比较,两烫伤组间同时进行比较。结果各组小鼠毛发颜色和生长发育无明显差异。野生型烫伤组心脏ATGL蛋白表达显著升高(1.00±0.68比3.09±0.93,P=0.023),而MHC-ATGL烫伤组心脏ATGL蛋白表达明显下降(17.84±2.41比10.36±2.22,P<0.001),但MHC-ATGL烫伤组心脏ATGL蛋白表达仍显著高于野生型烫伤组(P<0.001)。野生型烫伤组和MHC-ATGL烫伤组血清心肌肌钙蛋白T、血清肌酸激酶同工酶均显著高于其对照组[(0.456±0.131)比(0.076±0.019)μg/L和(0.219±0.089)比(0.060±0.019)μg/L、(1421±162)比(221±67)U/L和(761±142)比(221±41)U/L](均P<0.001),而MHC-ATGL烫伤组血清心肌肌钙蛋白T、血清肌酸激酶同工酶均显著低于野生型烫伤组(均P<0.001);野生型烫伤组心脏组织游离脂肪酸含量显著高于其对照组[(2.54±0.51)比(0.46±0.27)mmol/L](P<0.001),MHC-ATGL烫伤组无明显变化[(0.81±0.38)比(0.59±0.25)mmol/L](P=0.251),而MHC-ATGL烫伤组心脏游离脂肪酸含量显著低于野生型烫伤组(P<0.001)。野生型烫伤组和MHC-ATGL烫伤组心脏组织活性氧水平均显著高于其对照组[(1.89±0.23)比(1.00±0.18)和(1.38±0.17)比(0.95±0.13)](均P<0.001),而MHC-ATGL烫伤组心脏组织活性氧水平显著低于野生型烫伤组(P<0.001)。结论心脏过表达ATGL可能通过降低游离脂肪
Objective To explore the effect and potential mechanism of cardiac adipose triglyceride lipase(ATGL)overexpression on burn-induced cardiac injury.Methods Eight-week-old C57BL/6J mice with cardiac ATGL overexpression driven by the myosin heavy chain(MHC)promoter(MHC-ATGL burn group)and wild-type(wild-type burn group)mice were randomly chose to the following experiments with burn injury after 24 h(n=8/group),MHC-ATGL mice and wild-type mice with corresponded age and sex were included as control.Cardiac ATGL protein expression,serum levels of cardiac troponin T and cardiac kinase-MB(CK-MB),cardiac free fatty acid and reactive oxygen species were detected.The wild-type and MHC-ATGL burn groups were not only compared with their corresponded control groups,but also compared between each other.Results The hair color and development were shown little difference between each group.ATGL protein expression is elevated in wild-type burn group(1.00±0.68 vs 3.09±0.93,P=0.023)and decreased in MHC-ATGL burn group(17.84±2.41 vs 10.36±2.22,P<0.001),while ATGL protein expression is still increased in MHC-ATGL burn group compared with wild-type burn group(P<0.001).Serum levels of cardiac troponin T and CK-MB were both elevated in wild-type burn group and MHC-ATGL burn group[(0.456±0.131)vs(0.076±0.019)μg/L and(0.219±0.089)vs(0.060±0.019)μg/L,(1421±162)vs(221±67)U/L and(761±142)vs(221±41)U/L](all P<0.001),while serum levels of cardiac troponin T and CK-MB was still decreased in MHC-ATGL burn group compared with wild-type burn group(P<0.001).In addition,cardiac free fatty acid was increased in wild-type burn group and little difference was found in MHC-ATGL burn group[(2.54±0.51)vs(0.46±0.27)mmol/L,P<0.001,and(0.81±0.38)vs(0.59±0.25)mmol/L,P=0.251],while cardiac free fatty acid was significant reduction in MHC-ATGL burn group compared with wild-type burn group(P<0.001).Levels of cardiac reactive oxygen species was both elevated in wild-type burn group and MHC-ATGL burn group[(1.89±0.23)vs(1.00±0.18)and(1.38±0.17)v
作者
李凌霏
张琼
张星月
张均辉
冯燕海
吕艳玲
贾杰只
黄跃生
Li Lingfei;Zhang Qiong;Zhang Xingyue;Zhang Junhui;Feng Yanhai;Lv Yanling;Jia Jiezhi;Huang Yuesheng(Department of Dermatology,Daping Hospital,Third Military Medical University(Army Medical University),Chongqing,400042,China;Institute of Burn Research,State Key Laboratory of Trauma,Burns and Combined Injury,Southwest Hospital,Third Military Medical University(Army Medical University),Chongqing,400038,China)
出处
《中华医学杂志》
CAS
CSCD
北大核心
2020年第12期910-914,共5页
National Medical Journal of China
基金
国家自然科学基金面上项目(81671913)。
关键词
烧伤
脂肪酶
心肌损害
脂肪酸类
非酯化
活性氧
Burns
Adipose triglyceride lipase
Cardiac injury
Fatty acids
nonesterified
Reactive oxygen species
