摘要
试验旨在探讨PRKAA 2基因在摩拉水牛群体中的遗传多态性及其与生长性状的关联性,进而得到显著相关的遗传标记,为摩拉水牛的分子标记辅助选择提供理论依据。利用DNA测序法等技术进行候选基因PRKAA 2外显子4及部分内含子3单核苷酸多态性(SNPs)检测,并采用生物信息学方法结合统计软件分析PRKAA 2基因与摩拉水牛生长性状的关联性。结果显示,摩拉水牛PRKAA 2基因中共检测到4个SNPs位点:c.462 G>A、IVS3.557 T>C、IVS3.560 C>T和IVS3.565 G>A,均包含3种基因型,且符合Hardy-Weinberg平衡。4个SNPs位点在水牛群体中均为中度多态,可以构建3种单倍型,T-C-G-G为优势单倍型,其中IVS3.560 C>T与c.462 G>A位点之间存在完全连锁不平衡,关联分析结果显示,单倍型H2与摩拉水牛体斜长和腰角宽呈显著相关(P<0.05)。c.462 G>A位点与摩拉水牛体高、十字部高、尻长、坐骨端宽和腰角宽呈显著相关,其中GG和AA基因型个体的体高和十字部高均显著高于GA基因型个体,GG基因型个体的尻长显著高于GA和AA基因型个体,AA基因型个体的坐骨端宽和腰角宽显著高于GA基因型个体(P<0.05);IVS3.557 T>C位点与摩拉水牛的生长性状指标未达到显著相关(P>0.05);IVS3.560 C>T位点与摩拉水牛体高、十字部高和尻长呈显著相关,其中CC和TT基因型个体的体高和十字部高均显著高于CT基因型个体,CC基因型个体的尻长显著高于CT基因型个体(P<0.05);IVS3.565 G>A位点与体斜长呈显著相关,其中GG和GA基因型个体的体斜长显著高于AA基因型个体(P<0.05)。综上,PRKAA 2基因的c.462 G>A、IVS3.560 C>T和IVS3.565 G>A位点对摩拉水牛部分生长性状均有显著影响,可作为摩拉水牛品种早期选育的候选基因和分子辅助标记。
This study was aimed to investigate the genetic polymorphisms of PRKAA 2 gene in Murrah buffalo and its associations with growth traits,which could help to mine significant genetic markers for molecular marker assisted selection of Murrah buffalo.SNPs located in exon 4 and intron 3 were detected in PRKAA 2 gene of Murrah buffalo by random DNA sequencing method.Genetic variation of SNPs and the association of different genotypes with growth traits of Murrah buffalo were analyzed by bioinformatics method and statistical software.The results showed that 4 SNPs were detected in PRKAA 2 gene,namely c.462 G>A,IVS3.557 T>C,IVS3.560 C>T and IVS3.565 G>A,respectively,there were 3 genotypes.The fitness test showed that PRKAA 2 gene on polymorphic loci in Hardy-Weinberg equilibrium.All of 4 SNPs were moderate polymorphism,and could be constructed 3 haplotypes,T-C-G-G type was the dominant haplotype in Murrah buffalo.There was a complete linkage disequilibrium between IVS3.560 C>T and c.462 G>A.Association analysis results indicated that H2 haplotype was significantly correlated with body length(BL)and hip width(HW)in Murrah buffaloes(P<0.05).c.462 G>A was significantly correlated with the withers height(WH),height at hip cross(HHC),rump length(RL),pin bone width(PBW)and HW of Murrah buffalo,WH and HHC of GG and AA genotypes were significantly higher than that of GA genotype,RL of GG genotype were significantly higher than that of GA and AA genotypes,and PBW and HW of AA genotype were significantly higher than that of GA genotypes(P<0.05).There was no significant correlation between IVS3.557 T>C and body size traits of Murrah buffalo(P>0.05).IVS3.560 C>T was significantly correlated with WH,HHC and RL of Murrah buffalo,WH and HHC of CC and TT genotypes were significantly higher than that of CT genotype(P<0.05),and RL of CC genotype was significantly higher than that of CT genotype(P<0.05).IVS3.565 G>A was significantly correlated with BL of Murrah buffalo,BL of GG and GA genotypes were significantly higher than that of AA ge
作者
郑海英
杨春艳
郑威
于农淇
文崇利
韦科龙
李玲玉
黄春丽
黄加祥
尚江华
ZHENG Haiying;YANG Chunyan;ZHENG Wei;YU Nongqi;WEN Chongli;WEI Kelong;LI Lingyu;HUANG Chunli;HUANG Jiaxiang;SHANG Jianghua(Key Laboratory of Buffalo Genetics,Breeding and Reproduction Technology,Ministry of Agriculture and Rural Affairs,Guangxi Buffalo Research Institute,Chinese AcademyAgricultural Sciences,Nanning 530001,China)
出处
《中国畜牧兽医》
CAS
北大核心
2020年第3期789-798,共10页
China Animal Husbandry & Veterinary Medicine
基金
国家重点研发计划(2017YFE0113800)
广西科技计划项目(桂科AB16380040)
广西自然科学基金(2018GXNSFDA050013)
广西水产畜牧兽医局科技项目(桂渔牧科201452029、桂渔牧科201528015)。
